Frommolt Ruth, Rohrbach Florian, Theobald Matthias
Department of Hematology & Oncology, Johannes Gutenberg University of Mainz, Langenbeckstr. 1, 55101 Mainz, Germany.
Future Oncol. 2006 Jun;2(3):345-9. doi: 10.2217/14796694.2.3.345.
The Sleeping Beauty (SB) transposon system can mediate stable gene transfer and expression in primary human T cells. Optimal in vitro conditions for maximum gene transfer efficiencies have been developed with regard to further application of the SB transposon system in T cell based gene therapies. This raises the question of whether or not the SB transposon system is a convincing alternative for virus-mediated gene transfer based on the currently available data. Here, we will discuss controversial safety and efficacy issues in transposon and viral gene transfer technology.
睡美人（SB）转座子系统可介导基因在原代人T细胞中的稳定转移和表达。关于SB转座子系统在基于T细胞的基因治疗中的进一步应用，已开发出实现最大基因转移效率的最佳体外条件。基于目前可得的数据，这就引发了一个问题，即SB转座子系统是否是病毒介导基因转移的一个令人信服的替代方案。在此，我们将讨论转座子和病毒基因转移技术中存在争议的安全性和有效性问题。
