关于大肠杆菌中隔膜形成的控制
On the control of septation in Escherichia coli.
作者信息
Pages J M, Piovant M, Lazdunski A, Lazdunski C
出版信息
Biochimie. 1975;57(3):303-13. doi: 10.1016/s0300-9084(75)80306-3.
Mutants of E. coli defective in cell septation (ftsA to ftsG, conditional thermosensitive mutants isolated by Ricard and Hirota) were studied with respect to their membrane protein composition, murein hydrolase activities and rates of synthesis of murein and phospholipids. Three classes of mutants have been distinguished: 1) those affected in both murein and phospholipid synthesis; 2) those affected in either murein or phospholipid synthesis and 3) those affected in neither of these parameters. Overall murein hydrolase activities, after activation, is of the same order in all the mutants screened. In addition to soluble products of murein splitting, we have found insoluble products that appear to be in dynamic equilibrium with the murein of the sacculus. Endogenous levels of cyclic adenosine 3',5'-monophosphate measured after blocking septation showed no variation. This suggests that the cyclic nucleotide is not involved in the metabolic control of septation.
研究了大肠杆菌中细胞分裂缺陷型突变体（ftsA至ftsG，由里卡尔德和广田分离得到的条件性热敏突变体）的膜蛋白组成、胞壁质水解酶活性以及胞壁质和磷脂的合成速率。已区分出三类突变体：1）胞壁质和磷脂合成均受影响的突变体；2）胞壁质或磷脂合成受影响的突变体；3）这些参数均未受影响的突变体。在所有筛选的突变体中，激活后的总体胞壁质水解酶活性处于同一水平。除了胞壁质裂解的可溶性产物外，我们还发现了不溶性产物，它们似乎与细胞壁的胞壁质处于动态平衡。阻断细胞分裂后测得的环腺苷酸3',5'-单磷酸的内源性水平没有变化。这表明环核苷酸不参与细胞分裂的代谢控制。
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