Burman L G, Raichler J, Park J T
J Bacteriol. 1983 Sep;155(3):983-8. doi: 10.1128/jb.155.3.983-988.1983.
High-resolution autoradiography of thin sections of Escherichia coli cells whose murein was pulse-labeled with [3H]diaminopimelic acid after a period of diaminopimelic acid deprivation indicated that elongation of the murein sacculus occurs by a multisite (diffuse) process. Upon chasing, radioactivity in polar murein was stable, whereas radioactivity in cylindrical murein was reduced, indicating that diffuse intercalation of new murein occurred during cell elongation. Elongation and septation were shown to be overlapping processes.
在用二氨基庚二酸剥夺一段时间后,用[3H]二氨基庚二酸对其胞壁质进行脉冲标记的大肠杆菌细胞薄片的高分辨率放射自显影表明,胞壁质囊的伸长是通过多部位(弥散)过程发生的。追踪时,极性胞壁质中的放射性是稳定的,而圆柱形胞壁质中的放射性降低,这表明在细胞伸长过程中发生了新胞壁质的弥散插入。伸长和隔膜形成被证明是重叠的过程。