Kumar S, Thomas A, Pillai M K
Department of Zoology, University of Delhi, India.
Indian J Exp Biol. 1991 Apr;29(4):379-84.
Role of mono-oxygenases as a mechanism of resistance to the synthetic pyrethroid, deltamethrin in the larvae of Culex quinquefasciatus Say, Aedes aegypti L. and Anopheles stephensi Liston developed by laboratory selections with deltamethrin, DDT or deltamethrin and the synergist, piperonyl butoxide (PBO) in the ratio of 1:5, was investigated. There was a significant correlation with mono-oxygenase activity and larval LC50 to deltamethrin in various strains of all the three species. In addition, the activity of glucose-6-phosphate dehydrogenase (G6PD), the main NADPH generating enzyme for mono-oxygenases, also showed enhanced activity in deltamethrin and DDT-selected strains. The present data, therefore, clearly suggest that deltamethrin resistance in the larvae of Cx. quinquefasciatus, Ae. aegypti and An. stephensi is mainly due to the detoxification of deltamethrin by microsomal mono-oxygenases. High activity of G6PD observed in DDT-selected strains seems to be related to its role as a rate-limiting enzyme in GSH-dependent dehydrochlorination of DDT.
研究了单加氧酶作为致倦库蚊、埃及伊蚊和斯氏按蚊幼虫对拟除虫菊酯溴氰菊酯产生抗性的一种机制的作用，这些幼虫是通过用溴氰菊酯、滴滴涕或溴氰菊酯与增效剂胡椒基丁醚（PBO）按1:5的比例进行实验室选育得到的。在这三个物种的各种品系中，单加氧酶活性与幼虫对溴氰菊酯的半数致死浓度（LC50）之间存在显著相关性。此外，作为单加氧酶主要的烟酰胺腺嘌呤二核苷酸磷酸（NADPH）生成酶的葡萄糖-6-磷酸脱氢酶（G6PD）的活性，在溴氰菊酯和滴滴涕选育的品系中也表现出增强。因此，目前的数据清楚地表明，致倦库蚊、埃及伊蚊和斯氏按蚊幼虫对溴氰菊酯的抗性主要是由于微粒体单加氧酶对溴氰菊酯的解毒作用。在滴滴涕选育的品系中观察到的G6PD高活性似乎与其作为滴滴涕谷胱甘肽依赖性脱氯化氢反应中的限速酶的作用有关。
