Jagadeshwaran Urmila, Vijayan V A
School of Biological Sciences, Washington State University, Pullman, WA, USA.
Parasitol Res. 2009 Jun;104(6):1431-8. doi: 10.1007/s00436-009-1342-z. Epub 2009 Feb 19.
A resistant strain (DR) of Aedes aegypti, generated by deltamethrin selection for 20 consecutive generations from a laboratory susceptible strain (DS) was studied for the possible resistant mechanisms. The pyrethroid resistance developed was characterized by biochemical assays and native polyacrylamide gel electrophoresis. Significant elevation in the activity of alpha- and beta-esterases, glucose-6-phosphate dehydrogenase (G6PD), CYTP450 (CYTP450), and glutathione-s-transferase (GST) were noticed in DR. The gel profiles for esterases, G6PD, and CYTP450 were different in DR as compared to DS strain. The difference was either in the form of additional bands or increased intensity of the bands or both. Gel profile variations were also evident from densitometry. Our study suggests that these enzymes play an important role in deltamethrin resistance in the DR strain.
从实验室敏感品系（DS）连续20代用溴氰菊酯选育出的埃及伊蚊抗性品系（DR），对其可能的抗性机制进行了研究。通过生化分析和天然聚丙烯酰胺凝胶电泳对产生的拟除虫菊酯抗性进行了表征。在DR中发现α-和β-酯酶、葡萄糖-6-磷酸脱氢酶（G6PD）、细胞色素P450（CYTP450）和谷胱甘肽-S-转移酶（GST）的活性显著升高。与DS品系相比，DR中酯酶、G6PD和CYTP450的凝胶图谱不同。差异表现为出现额外条带、条带强度增加或两者兼有。通过光密度测定法也可明显看出凝胶图谱的变化。我们的研究表明，这些酶在DR品系对溴氰菊酯的抗性中起重要作用。
