Seve M, Chimienti F, Devergnas S, Aouffen M, Douki T, Chantegrel J, Cadet J, Favier A
Laboratoire des Lésions des Acides Nucléiques, DRFMC/SCIB, CEA/Grenoble, Grenoble, France.
Med Chem. 2005 Nov;1(6):629-33. doi: 10.2174/157340605774598144.
Resveratrol, a polyphenolic phytoalexin, is a very effective antioxidant that also exhibits strong antiproliferative and anti-inflammatory properties. Recent studies have provided support for the use of resveratrol in human cancer chemoprevention, in combination with either chemotherapeutic drugs or cytotoxic factors for a most efficient treatment of drug refractory tumor cells. Resveratrol is also widely used in topical preparations, as a chemoprotective compound against development of several cutaneous disorders, including skin cancer. Nevertheless, the combined effect of resveratrol and UVA irradiation on cellular toxicity and DNA damage has never been assessed. The aim of this work was to investigate the effect of resveratrol on cell fate in immortalized human keratinocytes HaCaT cells. The results indicated that resveratrol potentiates the production of significant amounts of 8-oxo-7,8-dihydro-2'-deoxyguanosine in UVA-irradiated genomic DNA. Moreover, the combination of resveratrol with UVA significantly enhances the induction of DNA strand breaks and cell death in HaCaT keratinocytes. The conclusion is a potential hazardous effect of topical application of resveratrol, particularly on regions exposed to sunlight.
白藜芦醇是一种多酚类植物抗毒素，是一种非常有效的抗氧化剂，还具有很强的抗增殖和抗炎特性。最近的研究为白藜芦醇用于人类癌症化学预防提供了支持，它可与化疗药物或细胞毒性因子联合使用，以最有效地治疗耐药肿瘤细胞。白藜芦醇还广泛用于局部制剂中，作为一种化学保护化合物，可预防包括皮肤癌在内的几种皮肤疾病的发生。然而，白藜芦醇与紫外线A辐射对细胞毒性和DNA损伤的联合作用从未被评估过。这项工作的目的是研究白藜芦醇对永生化人角质形成细胞HaCaT细胞命运的影响。结果表明，白藜芦醇可增强紫外线A照射的基因组DNA中大量8-氧代-7,8-二氢-2'-脱氧鸟苷的产生。此外，白藜芦醇与紫外线A的组合可显著增强HaCaT角质形成细胞中DNA链断裂和细胞死亡的诱导。结论是局部应用白藜芦醇可能具有有害作用，尤其是在暴露于阳光的区域。
