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白藜芦醇和泼尼松龙处理的人T细胞急性淋巴细胞白血病细胞系CCRF-CEM中[具体物质]的表达及凋亡研究 。 （原文中“Investigation of and expression”部分表述不完整，推测是有两种物质未完整写出，这里按照现有内容尽量准确翻译）

Investigation of and expression and apoptosis in a resveratrol- and prednisolone-treated human T-ALL cell line, CCRF-CEM.

作者信息

Khanzadeh Taghi, Hagh Majid Farshdousti, Talebi Mehdi, Yousefi Bahman, Azimi Ako, Hossein Pour Feizi Abbas Ali, Baradaran Behzad

机构信息

Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Blood Res. 2018 Mar;53(1):53-60. doi: 10.5045/br.2018.53.1.53. Epub 2018 Mar 27.

DOI:10.5045/br.2018.53.1.53
PMID:29662863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5898995/
Abstract

BACKGROUND

The numerous side effects and chemo-resistance of conventional chemical drugs in the treatment of malignancies have led to consideration of the anti-cancer properties of natural products. In the present study, we aimed to explore the apoptotic effect of two natural components, resveratrol and prednisolone, on the T acute lymphoblastic leukemia (ALL) cell line, CCRF-CEM. Our findings suggested the incorporation of these natural agents into drug regimens to treat patients with ALL.

METHODS

In this study, we investigated the effect of different doses of resveratrol (15, 50 and 100 µM) and prednisolone (700 µM) on (apoptosis promoter) and (apoptosis inhibitor) expressions following 24 and 48 hours of treatment on CCRF-CEM cells, using real-time PCR, and on apoptosis induction using flow cytometry.

RESULTS

The results showed a time- and dose-dependent increase in expression and a decrease in expression. Apoptosis was induced in CCRF-CEM cells treated with resveratrol and prednisolone for 24 and 48 hours. Combined resveratrol and prednisolone treatment showed synergistic effects on the overexpression of and the downregulation of . The drug combination had a greater influence on apoptosis induction compared with either drug administered alone after 48 hours of treatment.

CONCLUSION

The results of this study suggested that resveratrol exhibited a remarkable efficacy to improve the influence of glucocorticoids drugs, especially prednisolone, to induce apoptosis in the CCRF-CEM cell line.

摘要

背景

传统化学药物在治疗恶性肿瘤时存在众多副作用和化疗耐药性，这促使人们考虑天然产物的抗癌特性。在本研究中，我们旨在探讨两种天然成分白藜芦醇和泼尼松龙对T急性淋巴细胞白血病（ALL）细胞系CCRF-CEM的凋亡作用。我们的研究结果表明，可将这些天然药物纳入治疗ALL患者的药物方案中。

方法

在本研究中，我们使用实时聚合酶链反应（PCR），研究了不同剂量的白藜芦醇（15、50和100微摩尔）和泼尼松龙（700微摩尔）在处理CCRF-CEM细胞24小时和48小时后对（凋亡促进剂）和（凋亡抑制剂）表达的影响，以及使用流式细胞术对凋亡诱导的影响。

结果

结果显示，表达呈时间和剂量依赖性增加，表达减少。用白藜芦醇和泼尼松龙处理24小时和48小时的CCRF-CEM细胞诱导了凋亡。白藜芦醇和泼尼松龙联合治疗对的过表达和的下调显示出协同作用。与单独使用任一药物相比，药物组合在处理48小时后对凋亡诱导的影响更大。

结论

本研究结果表明，白藜芦醇在提高糖皮质激素药物（尤其是泼尼松龙）诱导CCRF-CEM细胞系凋亡的影响方面表现出显著疗效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/007941f5b6ba/br-53-53-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/f899dfc079b6/br-53-53-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/19dc32b8a52a/br-53-53-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/89d379bae2d6/br-53-53-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/753308b15722/br-53-53-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/007941f5b6ba/br-53-53-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/f899dfc079b6/br-53-53-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/19dc32b8a52a/br-53-53-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/89d379bae2d6/br-53-53-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/753308b15722/br-53-53-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e78/5898995/007941f5b6ba/br-53-53-g005.jpg

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