Byrne D J, Phillips G, Napier A, Cuschieri A
Department of Surgery, Ninewells Hospital and Medical School, Dundee, Scotland.
J Hosp Infect. 1991 Jun;18(2):145-8. doi: 10.1016/0195-6701(91)90159-6.
As part of a large whole body disinfection (WBD) trial two small sub-groups of patients who showered preoperatively with either a 4% chlorhexidine (CHX: N = 29) or placebo (N =27) detergent were studied to assess intraoperative wound contamination. The groups were well matched for age, sex and length of surgery. A membrane filter contact technique was used for bacterial recovery from the wounds after the initial skin incision and before wound closure. The membrane filters were incubated aerobically on blood agar plates with a CHX neutralizer for 48 h at 37 degrees C and colonies were counted. The results show a significant difference, between the bacterial counts at the start and end of surgery in the CHX and placebo groups. There was no difference in bacterial counts at the start of surgery between the CHX and placebo groups. There was a significant difference in the bacterial counts at the end of surgery between the CHX and placebo groups. These results indicate that preoperative WBD with CHX reduces intraoperative wound contamination but the effect of this on postoperative wound sepsis rates awaits the results of a large WBD trial.
作为一项大型全身消毒（WBD）试验的一部分，对两个小亚组患者进行了研究，这两个亚组患者术前分别使用4%氯己定（CHX：N = 29）或安慰剂（N = 27）洗涤剂进行淋浴，以评估术中伤口污染情况。两组在年龄、性别和手术时长方面匹配良好。采用膜滤器接触技术在初次皮肤切口后和伤口闭合前从伤口处回收细菌。将膜滤器在含有CHX中和剂的血琼脂平板上于37℃需氧培养48小时，然后对菌落进行计数。结果显示，CHX组和安慰剂组手术开始和结束时的细菌计数存在显著差异。CHX组和安慰剂组手术开始时的细菌计数无差异。CHX组和安慰剂组手术结束时的细菌计数存在显著差异。这些结果表明，术前使用CHX进行全身消毒可减少术中伤口污染，但这对术后伤口败血症发生率的影响有待大型全身消毒试验的结果。
