Soria José Miguel, Martínez Ramos Cristina, Salmerón Sánchez Manuel, Benavent Veronique, Campillo Fernández Alberto, Gómez Ribelles José Luis, García Verdugo José Manuel, Pradas Manuel Monleón, Barcia Juan Antonio
Fundación Hospital General Universitario de Valencia, Avda Tres Cruces s/n, 46014 Valencia, Spain.
J Biomed Mater Res A. 2006 Dec 1;79(3):495-502. doi: 10.1002/jbm.a.30803.
Biomaterials prepared from polyacrylamide, ethyl acrylate (EA), and hydroxyethyl acrylate (HEA) in various blend ratios, methyl acrylate and chitosan, were tested in vitro as culture substrates and compared for their ability to be colonized by the cells migrating from embryonic brain explants. Neural explants were isolated from proliferative areas of the medial ganglionic eminence and the cortical ventricular zone of embryonic rat brains and cultured in vitro on the different biomaterials. Chitosan, poly(methyl acrylate), and the 50% wt copolymer of EA and HEA were the most suitable substrates to promote cell attachment and differentiation of the neural cells among those tested. Immunofluorescence microscopy analysis showed that progenitor cells had undergone differentiation and that the resulting glial and neuronal cells expressed their intrinsic morphological characteristics in culture.
