Center for Biomaterials and Tissue Engineering, Universidad Politécnica de Valencia, Camino de Vera, Spain.
J Biomater Appl. 2011 Sep;26(3):293-310. doi: 10.1177/0885328210365005. Epub 2010 Jun 21.
Biomaterials based on the hydrophobic homopolymer poly(ethyl acrylate), PEA, and its copolymers with hydroxyethyl acrylate, p(EA-co-HEA) and methacrylic acid, p(EA-co-MAAc) were prepared as polymeric scaffolds with interconnected pores of 90 microns and tested in vitro as culture substrates and compared for their impact on the differentiation of neural stem cells (NSC) obtained from the subventricular zone (SVZ) of postnatal rats and human endothelial cells (HUVEC). Immunocytochemical staining assay for specific markers show that p(EA-co-MAAc) scaffolds were suitable substrates to promote cell attachment and differentiation of adult NSC and HUVEC cells.
基于疏水性均聚物聚（丙烯酸乙酯）（PEA）及其与羟乙基丙烯酸酯（p(EA-co-HEA)）和甲基丙烯酸（p(EA-co-MAAc)）的共聚物的生物材料被制备为具有 90 微米连通孔的聚合物支架，并在体外作为培养底物进行测试，并比较它们对源自新生大鼠室下区（SVZ）和人内皮细胞（HUVEC）的神经干细胞（NSC）分化的影响。特定标志物的免疫细胞化学染色分析表明，p(EA-co-MAAc)支架是促进成年 NSC 和 HUVEC 细胞附着和分化的合适底物。
