动物病原体样III型分泌系统是鼻疽伯克霍尔德菌在J774.2巨噬细胞内存活所必需的。
The animal pathogen-like type III secretion system is required for the intracellular survival of Burkholderia mallei within J774.2 macrophages.
作者信息
Ribot Wilson J, Ulrich Ricky L
机构信息
Bacteriology Division, USAMRIID, 1425 Porter St., Fort Detrick, Frederick, MD 21702, USA.
出版信息
Infect Immun. 2006 Jul;74(7):4349-53. doi: 10.1128/IAI.01939-05.
Abstract
Burkholderia mallei is a highly infectious gram-negative pathogen and is the causative agent of human and animal glanders. By generating polar mutations (disruption of bsaQ and bsaZ) in the B. mallei ATCC 23344 animal pathogen-like type III secretion system (TTS), we demonstrate that this bacterial protein delivery system is required for intracellular growth of B. mallei in J774.2 cells, formation of macrophage membrane protrusions, actin polymerization, and phagosomal escape. These findings suggest that TTS plays a role in the intracellular trafficking of B. mallei and may facilitate cell-to-cell spread via actin-based motility.
摘要
鼻疽伯克霍尔德菌是一种极具传染性的革兰氏阴性病原体，是人类和动物鼻疽病的病原体。通过在鼻疽伯克霍尔德菌ATCC 23344动物病原体样III型分泌系统（TTS）中产生极性突变（bsaQ和bsaZ的破坏），我们证明这种细菌蛋白递送系统是鼻疽伯克霍尔德菌在J774.2细胞内生长、巨噬细胞膜突起形成、肌动蛋白聚合和吞噬体逃逸所必需的。这些发现表明，TTS在鼻疽伯克霍尔德菌的细胞内运输中发挥作用，并可能通过基于肌动蛋白的运动促进细胞间传播。
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