Reif J C, Warburton M L, Xia X C, Hoisington D A, Crossa J, Taba S, Muminović J, Bohn M, Frisch M, Melchinger A E
Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, 70593 Stuttgart, Germany.
Theor Appl Genet. 2006 Jul;113(2):177-85. doi: 10.1007/s00122-006-0283-5. Epub 2006 Apr 22.
Mexican races of maize (Zea mays L.) represent a valuable genetic resource for breeding and genetic surveys. We applied simple sequence repeat (SSR) markers to characterize 25 accessions of races of maize from Mexico. Our objectives were to (1) study the molecular genetic diversity within and among these accessions and (2) examine their relationships as assumed previously on the basis of morphological data. A total of 497 individuals were fingerprinted with 25 SSR markers. We observed a high total number of alleles (7.84 alleles per locus) and total gene diversity (0.61), confirming the broad genetic base of the maize races from Mexico. In addition, the accessions were grouped into distinct racial complexes on the basis of a model-based clustering approach. The principal coordinate analyses of the four Modern Incipient hybrids corroborated the proposed parental races of Chalqueño, Cónico Norteño, Celaya, and Bolita on the basis of the morphological data. Consequently, for some of the accessions, hybridizations provide a clue that can further be used to explain the associations among the Mexican races of maize.
墨西哥玉米品种（Zea mays L.）是育种和基因调查的宝贵遗传资源。我们应用简单序列重复（SSR）标记对来自墨西哥的25个玉米品种进行特征分析。我们的目标是：（1）研究这些品种内部和之间的分子遗传多样性；（2）根据之前基于形态学数据所假定的关系，检验它们之间的亲缘关系。用25个SSR标记对总共497个个体进行了指纹图谱分析。我们观察到等位基因总数很高（每个位点7.84个等位基因），总基因多样性为0.61，这证实了墨西哥玉米品种具有广泛的遗传基础。此外，基于基于模型的聚类方法，这些品种被分为不同的种族复合体。对四个现代初始杂种的主坐标分析证实了基于形态学数据所提出的查尔克尼奥、北圆锥体、塞拉亚和博利塔的亲本品种。因此，对于一些品种来说，杂交提供了一条线索，可进一步用于解释墨西哥玉米品种之间的关联。
