在正常大鼠活体脑内，可通过局部质子核磁共振波谱法检测脑葡萄糖。

Cerebral glucose is detectable by localized proton NMR spectroscopy in normal rat brain in vivo.

作者信息

Gyngell M L, Michaelis T, Hörstermann D, Bruhn H, Hänicke W, Merboldt K D, Frahm J

机构信息

Max-Planck-Institut für biophysikalische Chemie, Göttingen, Federal Republic of Germany.

出版信息

Magn Reson Med. 1991 Jun;19(2):489-95. doi: 10.1002/mrm.1910190243.

DOI:10.1002/mrm.1910190243

PMID:1679190

Abstract

This contribution reports the first direct and noninvasive observation of cerebral glucose in normal anesthetized rats (n = 16) using short-echo-time localized proton NMR spectroscopy (2.35 T, STEAM, TR = 6000 ms, TE = 20 ms, 125 microliters). In addition to resonances from N-acetyl aspartate (NAA), glutamate, total creatine, cholines, taurine, and myoinositol, all spectra exhibit strongly coupled resonances from glucose (3.43, 3.80 ppm) that are readily identifiable using model solutions. The observed level of cerebral glucose in fasted rats covered a range of 15-40% of that of NAA giving absolute concentrations of 1.1-2.8 mM when NAA is taken to be 7 mM. The arterial blood glucose concentration was 7.7 +/- 0.8 mM in the same group of animals.

摘要

本研究报告了使用短回波时间局部质子核磁共振波谱法（2.35 T，STEAM，TR = 6000 ms，TE = 20 ms，125微升）对正常麻醉大鼠（n = 16）进行脑葡萄糖的首次直接和非侵入性观察。除了来自N-乙酰天门冬氨酸（NAA）、谷氨酸、总肌酸、胆碱、牛磺酸和肌醇的共振外，所有光谱均显示出葡萄糖（3.43、3.80 ppm）的强耦合共振，使用模型溶液很容易识别。禁食大鼠中观察到的脑葡萄糖水平占NAA的15 - 40%，当NAA为7 mM时，绝对浓度为1.1 - 2.8 mM。同一组动物的动脉血糖浓度为7.7±0.8 mM。