Meyer Jens E, Stangenberg Stefanie, Weise Jan B, Beck Christian, Schmidt Christian, Kurz Kristina, Beier Ulf H, Maune Steffen
Department of Otorhinolaryngology, Head and Neck Surgery, University of Schleswig-Holstein, Campus Lübeck, Lübeck, Germany.
Rhinology. 2006 Jun;44(2):140-4.
Tissue eosinophilia is a hallmark of nasal polyposis and its pathogenesis is an area of high interest. RANTES and eotaxin are both known to recruit eosinophils, however, the mechanisms triggering their induction are still uncertain, and viral infections have been suggested to be involved in this process. Therefore, we investigated whether rhinovirus infection is a stimulus for RANTES and eotaxin expression and production. Fibroblasts were cultured from healthy nasal mucosa obtained during endonasal surgery. Cultured cells were infected with human rhinovirus-16 for one to 72 hours. Following total RNA isolation and reverse transcription, RANTES- and eotaxin-mRNA levels were analyzed. In addition, RANTES and eotaxin secretion was measured in culture supernatants by means of an ELISA. Rhinovirus infection induces RANTES-mRNA expression as early as one hour after infection, persisting for up to 72 hours. Eotaxin-mRNA profiles did not alter significantly from control. Protein production was confirmatory for both chemokines, indicating distinct translational latency. Our data suggest that RANTES functions as a host defence mechanism responding to rhinovirus infection, thus supporting a linkage between rhinovirus infections and the pathogenesis of nasal polyposis.
组织嗜酸性粒细胞增多是鼻息肉病的一个标志，其发病机制是一个备受关注的领域。已知RANTES和嗜酸性粒细胞趋化因子都能募集嗜酸性粒细胞，然而，触发它们诱导的机制仍不确定，并且有人提出病毒感染参与了这一过程。因此，我们研究了鼻病毒感染是否是RANTES和嗜酸性粒细胞趋化因子表达及产生的刺激因素。从鼻内手术中获取的健康鼻黏膜培养成纤维细胞。将培养的细胞用人鼻病毒16感染1至72小时。在进行总RNA分离和逆转录后，分析RANTES和嗜酸性粒细胞趋化因子的mRNA水平。此外，通过酶联免疫吸附测定法测量培养上清液中RANTES和嗜酸性粒细胞趋化因子的分泌。鼻病毒感染在感染后1小时就诱导RANTES的mRNA表达，持续长达72小时。嗜酸性粒细胞趋化因子的mRNA谱与对照相比没有显著变化。两种趋化因子的蛋白质产生得到了证实，表明存在明显的翻译延迟。我们的数据表明，RANTES作为一种宿主防御机制对鼻病毒感染作出反应，从而支持了鼻病毒感染与鼻息肉病发病机制之间的联系。
