Binz Sara K, Dickson Anne M, Haring Stuart J, Wold Marc S
Department of Biochemistry, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, USA.
Methods Enzymol. 2006;409:11-38. doi: 10.1016/S0076-6879(05)09002-6.
Replication protein A (RPA) is a heterotrimeric, single-stranded DNA-binding protein. RPA is conserved in all eukaryotes and is essential for DNA replication, DNA repair, and recombination. RPA also plays a role in coordinating DNA metabolism and the cellular response to DNA damage. Assays have been established for many of these reactions. This chapter provides an overview of the methods used for analyzing RPA-DNA interactions, RPA-protein interactions, and functional activities of RPA. Methods are also discussed for visualizing RPA in the cell and analyzing the effects of RPA function on cell cycle progression in mammalian cells.
复制蛋白A（RPA）是一种异源三聚体单链DNA结合蛋白。RPA在所有真核生物中都保守，对DNA复制、DNA修复和重组至关重要。RPA在协调DNA代谢和细胞对DNA损伤的反应中也发挥作用。已经针对许多这些反应建立了检测方法。本章概述了用于分析RPA-DNA相互作用、RPA-蛋白质相互作用以及RPA功能活性的方法。还讨论了在细胞中可视化RPA以及分析RPA功能对哺乳动物细胞细胞周期进程影响的方法。
