Colmin C, Pebay M, Simonet J M, Decaris B
Laboratoire de Génétique et Microbiologie, Université de Nancy I. Faculté des sciences, Vandoeuvre-lès-Nancy, France.
FEMS Microbiol Lett. 1991 Jun 15;65(2):123-8. doi: 10.1016/0378-1097(91)90290-q.
Genomic polymorphism in Streptococcus salivarius subsp. thermophilus was revealed by DNA restriction pattern analysis. A 4.2-kb variable DNA fragment was cloned from strain NST7 and hybridised with the DNA of 25 strains allowing an easy detection of intraspecific RFLP. Strong and weak hybridisation signals were observed and the latter were specifically revealed by a 2.1-kb fragment of the probe. Probe specificity was demonstrated by the absence of homology with DNA of strains belonging to 10 other species, with the exception of S. salivarius subsp. salivarius, confirming a close relationship between S. salivarius and S. thermophilus.
通过DNA限制性图谱分析揭示了嗜热唾液链球菌亚种的基因组多态性。从菌株NST7中克隆出一个4.2kb的可变DNA片段，并与25个菌株的DNA杂交，从而便于检测种内限制性片段长度多态性（RFLP）。观察到强杂交信号和弱杂交信号，后者由探针的一个2.1kb片段特异性显示。除唾液链球菌唾液亚种外，与其他10个物种的菌株DNA不存在同源性，证明了探针的特异性，证实了唾液链球菌和嗜热唾液链球菌之间的密切关系。
