用于口腔链球菌特异性鉴定的DNA探针的构建。
Construction of a DNA probe for the specific identification of Streptococcus oralis.
作者信息
Schmidhuber S, Ludwig W, Schleifer K H
机构信息
Lehrstuhl für Mikrobiologie, Technische Universität München, Federal Republic of Germany.
出版信息
J Clin Microbiol. 1988 May;26(5):1042-4. doi: 10.1128/jcm.26.5.1042-1044.1988.
Abstract
A DNA probe for the specific detection and identification of Streptococcus oralis was isolated from HindII-digested DNA of S. oralis NCTC 11427 and cloned in Escherichia coli. The plasmid pOS1 hybridized only to DNA of S. oralis and not to that of other viridans group streptococci.
摘要
从口腔链球菌NCTC 11427经HindII消化的DNA中分离出一种用于特异性检测和鉴定口腔链球菌的DNA探针，并将其克隆到大肠杆菌中。质粒pOS1仅与口腔链球菌的DNA杂交，而不与其他草绿色链球菌群的DNA杂交。
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