Wogan G N, Paglialunga S, Archer M C, Tannenbaum S R
Cancer Res. 1975 Aug;35(8):1981-4.
Carcinogenic activity of several synthetic N-nitroso compounds was evaluated in C57BL/6J X C3HeB/FeJ F1 mice. Test substances, suspended in trioctanoin, were injected i.p. in three equal doses given on Days 1, 4, and 7 after birth and animals were held without further treatment for up to 85 weeks. Nitrosoephedrine at a total dose of 600 mg/kg induced metastasizing liver cell carcinomas in 28 of 30 animals. Nitrososarcosine (225 mg/kg) induced similar tumors in 8 of 14 animals. Nitrosofolic acid (375 mg/kg) induced lung adenocarcinomas in 4 of 28 mice. Creatinine-5-oxime (600 mg/kg) showed no evidence of carcinogenic activity. Diethylnitrosamine (12 mg/kg given in four doses), included as a positive control, caused metastasizing liver cell tumors in 23 of 25 animals.
在C57BL/6J×C3HeB/FeJ F1小鼠中评估了几种合成N-亚硝基化合物的致癌活性。将受试物质悬浮于三辛精中，在出生后第1、4和7天腹腔注射三次等量剂量，动物不再接受进一步处理，观察长达85周。总剂量为600mg/kg的亚硝基麻黄碱在30只动物中的28只诱发了转移性肝细胞癌。亚硝基肌氨酸（225mg/kg）在14只动物中的8只诱发了类似肿瘤。亚硝基叶酸（375mg/kg）在28只小鼠中的4只诱发了肺腺癌。肌酐-5-肟（600mg/kg）未显示出致癌活性的证据。作为阳性对照的二乙基亚硝胺（分四次给予，每次12mg/kg）在25只动物中的23只诱发了转移性肝细胞肿瘤。
