Hano C, Martin I, Fliniaux O, Legrand B, Gutierrez L, Arroo R R J, Mesnard F, Lamblin F, Lainé E
Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, Antenne Scientifique Universitaire de Chartres, 21 rue de Loigny la Bataille, 28000, Chartres, France.
Planta. 2006 Nov;224(6):1291-301. doi: 10.1007/s00425-006-0308-y. Epub 2006 May 31.
The transcription activity of the pinoresinol-lariciresinol reductase (PLR) gene of Linum usitatissimum (so-called LuPLR), a key gene in lignan synthesis, was studied by RT-PCR and promoter-reporter transgenesis. The promoter was found to drive transcription of a GUSint reporter gene in the seed coats during the flax seed development. This fitted well with the tissue localization monitored by semi-quantitative RT-PCR of LuPLR expression. Accumulation of the main flax lignan secoisolariciresinol diglucoside was coherent with LuPLR expression during seed development. This three-way approach demonstrated that the LuPLR gene is expressed in the seed coat of flax seeds, and that the synthesis of PLR enzyme occurs where flax main lignan is found stored in mature seeds, confirming its involvement in SDG synthesis.
通过逆转录聚合酶链反应（RT-PCR）和启动子-报告基因转基因技术，对亚麻籽中木脂素合成关键基因——松脂醇-落叶松脂醇还原酶（PLR）基因（即LuPLR）的转录活性进行了研究。结果发现，该启动子在亚麻籽发育过程中驱动种皮中GUSint报告基因的转录。这与通过半定量RT-PCR监测LuPLR表达的组织定位结果非常吻合。在种子发育过程中，亚麻主要木脂素开环异落叶松脂醇二葡萄糖苷的积累与LuPLR表达一致。这种三管齐下的方法表明，LuPLR基因在亚麻籽种皮中表达，且PLR酶的合成发生在成熟种子中储存亚麻主要木脂素的部位，证实了其参与开环异落叶松脂醇二葡萄糖苷（SDG）的合成。
