Chen Y-W, Chiou S-H, Wong T-T, Ku H-H, Lin H-T, Chung C-F, Yen S-H, Kao C-L
Cancer Center, Department of Medical Research & Education, Taipei Veterans General Hospital, and Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
Transplant Proc. 2006 Jun;38(5):1616-7. doi: 10.1016/j.transproceed.2006.02.084.
Gelatin scaffolds for ex vivo cell cultures are a promising development. These scaffolds can be used as three-dimensional skeletons for cell attachment and culture before transplantation. In this study, we isolated and cultivated neural stem cells from human brain tissues in serum-free medium (DMEM+F12 nutrient). Better neuron growth was observed using the tetrazolium assay (MTT) in the group when basic fibroblast growth factor (bFGF) was coated on the gelatin polymer scaffold. Further development of this nontoxic system may help the future development of transplantation of human neural stem cells.
