组织工程：策略、干细胞与支架
Tissue engineering: strategies, stem cells and scaffolds.
作者信息
Howard Daniel, Buttery Lee D, Shakesheff Kevin M, Roberts Scott J
机构信息
Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Science, School of Pharmacy, University of Nottingham, UK.
出版信息
J Anat. 2008 Jul;213(1):66-72. doi: 10.1111/j.1469-7580.2008.00878.x. Epub 2008 Apr 15.
Abstract
Tissue engineering scaffolds are designed to influence the physical, chemical and biological environment surrounding a cell population. In this review we focus on our own work and introduce a range of strategies and materials used for tissue engineering, including the sources of cells suitable for tissue engineering: embryonic stem cells, bone marrow-derived mesenchymal stem cells and cord-derived mesenchymal stem cells. Furthermore, we emphasize the developments in custom scaffold design and manufacture, highlighting laser sintering, supercritical carbon dioxide processing, growth factor incorporation and zoning, plasma modification of scaffold surfaces, and novel multi-use temperature-sensitive injectable materials.
摘要
组织工程支架旨在影响细胞群体周围的物理、化学和生物环境。在本综述中，我们重点介绍我们自己的工作，并介绍一系列用于组织工程的策略和材料，包括适用于组织工程的细胞来源：胚胎干细胞、骨髓间充质干细胞和脐带间充质干细胞。此外，我们强调定制支架设计和制造方面的进展，突出激光烧结、超临界二氧化碳处理、生长因子掺入和分区、支架表面的等离子体改性以及新型多用途温度敏感可注射材料。
相似文献
1
Tissue engineering: strategies, stem cells and scaffolds.
J Anat. 2008 Jul;213(1):66-72. doi: 10.1111/j.1469-7580.2008.00878.x. Epub 2008 Apr 15.
2
[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Feb 15;32(2):215-222. doi: 10.7507/1002-1892.201708017.
3
Osteochondral tissue engineering: scaffolds, stem cells and applications.
J Cell Mol Med. 2012 Oct;16(10):2247-70. doi: 10.1111/j.1582-4934.2012.01571.x.
4
Adipose-derived mesenchymal stem cell seeded Atelocollagen scaffolds for cardiac tissue engineering.
J Mater Sci Mater Med. 2020 Sep 23;31(10):83. doi: 10.1007/s10856-020-06425-2.
5
Recellularization of Native Tissue Derived Acellular Scaffolds with Mesenchymal Stem Cells.
Cells. 2021 Jul 15;10(7):1787. doi: 10.3390/cells10071787.
6
Injectable degradable PVA microgels prepared by microfluidic technology for controlled osteogenic differentiation of mesenchymal stem cells.
Acta Biomater. 2018 Sep 1;77:28-37. doi: 10.1016/j.actbio.2018.07.003. Epub 2018 Jul 5.
7
Hydrogel fibers encapsulating human stem cells in an injectable calcium phosphate scaffold for bone tissue engineering.
Biomed Mater. 2016 Nov 4;11(6):065008. doi: 10.1088/1748-6041/11/6/065008.
8
Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering.
Int J Nanomedicine. 2011;6:3065-75. doi: 10.2147/IJN.S25297. Epub 2011 Nov 30.
9
Human mesenchymal stem cells cultured on silk hydrogels with variable stiffness and growth factor differentiate into mature smooth muscle cell phenotype.
Acta Biomater. 2016 Feb;31:156-166. doi: 10.1016/j.actbio.2015.11.051. Epub 2015 Nov 24.
10
PHBV wet-spun scaffold coated with ELR-REDV improves vascularization for bone tissue engineering.
Biomed Mater. 2018 Jul 27;13(5):055010. doi: 10.1088/1748-605X/aad139.
引用本文的文献
1
Strategies for the vascular patterning of engineered tissues for organ repair.
Nat Biomed Eng. 2025 Jun 20. doi: 10.1038/s41551-025-01420-w.
2
Regenerative Endodontic Therapies: Harnessing Stem Cells, Scaffolds, and Growth Factors.
Polymers (Basel). 2025 May 26;17(11):1475. doi: 10.3390/polym17111475.
3
analysis of hybrid hydrogels containing dual growth factor combinations, and skeletal stem cells under mechanical stimulation for bone repair.
Mechanobiol Med. 2024 Aug 27;2(4):100096. doi: 10.1016/j.mbm.2024.100096. eCollection 2024 Dec.
4
Engineered Tissue Models to Decode Host-Microbiota Interactions.
Adv Sci (Weinh). 2025 Jun;12(23):e2417687. doi: 10.1002/advs.202417687. Epub 2025 May 14.
5
Silk Fibroin Methacrylation: Chemical Synthesis to Biomechanical Optimization in Tissue Engineering.
ACS Biomater Sci Eng. 2025 Jun 9;11(6):3114-3125. doi: 10.1021/acsbiomaterials.4c01931. Epub 2025 May 9.
6
Biomaterials for Guided Tissue Regeneration and Guided Bone Regeneration: A Review.
Dent J (Basel). 2025 Apr 21;13(4):179. doi: 10.3390/dj13040179.
7
Molecular dynamics simulation in tissue engineering.
Bioimpacts. 2024 Aug 3;15:30160. doi: 10.34172/bi.30160. eCollection 2025.
8
Hydrogels for Cardiac Tissue Regeneration: Current and Future Developments.
Int J Mol Sci. 2025 Mar 5;26(5):2309. doi: 10.3390/ijms26052309.
9
DNA-encoded dynamic hydrogels for 3D bioprinted cartilage organoids.
Mater Today Bio. 2025 Jan 21;31:101509. doi: 10.1016/j.mtbio.2025.101509. eCollection 2025 Apr.
10
Crosslinker-free hydrogel induces self-aggregation of human dental pulp stem cells with enhanced antibacterial activity.
Mater Today Bio. 2025 Jan 4;31:101451. doi: 10.1016/j.mtbio.2025.101451. eCollection 2025 Apr.
本文引用的文献
1
Microtopography of metal surfaces influence fibroblast growth by modifying cell shape, cytoskeleton, and adhesion.
J Orthop Res. 2007 Nov;25(11):1523-33. doi: 10.1002/jor.20430.
2
Growth factors for clinical-scale expansion of human articular chondrocytes: relevance for automated bioreactor systems.
Tissue Eng. 2007 Jun;13(6):1227-34. doi: 10.1089/ten.2006.0342.
3
Clinical experience using cultured epithelial autografts leads to an alternative methodology for transferring skin cells from the laboratory to the patient.
Regen Med. 2006 Nov;1(6):809-21. doi: 10.2217/17460751.1.6.809.
4
Taking tissue-engineering principles into theater: augmentation of impacted allograft with human bone marrow stromal cells.
Regen Med. 2006 Sep;1(5):685-92. doi: 10.2217/17460751.1.5.685.
5
Three-Dimensional Bioactive and Biodegradable Scaffolds Fabricated by Surface-Selective Laser Sintering.
Adv Mater. 2004 Dec 20;17(3):327-330. doi: 10.1002/adma.200400838.
6
Development of a slow non-viral DNA release system from PDLLA scaffolds fabricated using a supercritical CO2 technique.
Biotechnol Bioeng. 2007 Oct 15;98(3):679-93. doi: 10.1002/bit.21446.
7
Cell adhesion and mechanical properties of a flexible scaffold for cardiac tissue engineering.
Acta Biomater. 2007 Jul;3(4):457-62. doi: 10.1016/j.actbio.2006.12.006. Epub 2007 Feb 26.
8
Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
Biotechnol Bioeng. 2007 Aug 15;97(6):1617-25. doi: 10.1002/bit.21343.
9
Improved human embryonic stem cell embryoid body homogeneity and cardiomyocyte differentiation from a novel V-96 plate aggregation system highlights interline variability.
Stem Cells. 2007 Apr;25(4):929-38. doi: 10.1634/stemcells.2006-0598. Epub 2006 Dec 21.
10
Zonal release of proteins within tissue engineering scaffolds.
J Mater Sci Mater Med. 2006 Nov;17(11):1049-56. doi: 10.1007/s10856-006-0443-9. Epub 2006 Nov 22.
Zotero 插件