Cao Xue-Li, Xu Ya-Tao, Zhang Guang-Ming, Xie Sheng-Meng, Dong Ying-Mao, Ito Yoichiro
Beijing Technology and Business University, School of Chemical and Environmental Engineering, Beijing Key Lab of Plant Resource Research and Development, Beijing 100037, China.
J Chromatogr A. 2006 Sep 15;1127(1-2):92-6. doi: 10.1016/j.chroma.2006.05.083. Epub 2006 Jun 22.
High-speed counter-current chromatography (HSCCC) is applied to the purification of coenzyme Q(10) (CoQ(10)) for the first time. CoQ(10) was obtained from a fermentation broth extract. A non-aqueous two-phase solvent system composed of heptane-acetonitrile-dichloromethane (12:7:3.5, v/v/v) was selected by analytical HSCCC and used for purification of CoQ(10) from 500 mg of the crude extract. The separation yielded 130 mg of CoQ(10) at an HPLC purity of over 99%. The overall results of the present studies show the advantages of HSCCC over an alternative of silica gel chromatography followed by recrystallization. These advantages extend to higher purity (97.8% versus 93.3%), recovery (88% versus 74.3%) and yield (26.4% versus 23.4%). An effort to avoid the toxic, expensive solvent CH(2)Cl(2) was unsuccessful, but at least its percentage is low in the solvent system.
高速逆流色谱法（HSCCC）首次应用于辅酶Q10（CoQ10）的纯化。CoQ10是从发酵液提取物中获得的。通过分析型HSCCC选择了由庚烷 - 乙腈 - 二氯甲烷（12:7:3.5，v/v/v）组成的非水两相溶剂系统，并用于从500mg粗提取物中纯化CoQ10。分离得到130mg CoQ10，HPLC纯度超过99%。本研究的总体结果表明HSCCC优于硅胶柱色谱法后重结晶的方法。这些优势体现在更高的纯度（97.8%对93.3%）、回收率（88%对74.3%）和产率（26.4%对23.4%）上。避免使用有毒、昂贵的溶剂CH2Cl2的尝试未成功，但至少其在溶剂系统中的比例较低。
