Urick M E, Johnson P A
Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.
Gynecol Oncol. 2006 Nov;103(2):673-8. doi: 10.1016/j.ygyno.2006.05.012. Epub 2006 Jun 22.
Our purpose was to determine the mRNA and protein expression of cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2) in ovarian tumors and normal ovaries of the hen, which is an excellent model for human ovarian cancer. Tissue concentrations of prostaglandin E(2) (PGE2) and PGE2 metabolites were also determined.
Tissue was obtained from ovarian tumor (n = 18) and normal ovary (n = 29) of 2- to 4-year old Single-comb White Leghorn hens. Quantitative real-time PCR with Sybr Green was used to quantify the mRNA expression of COX-1 and COX-2, using 18S expression as an internal control for COX normalization. Immunohistochemistry using antibodies for COX-1 and COX-2 was used to localize protein expression of each isoform in a subset of tumor (n = 5) and normal samples (n = 6). For determination of tissue prostaglandin concentration, tissue was obtained from ovarian tumor (n = 8) and normal ovary (n = 8). PGE2 and PGE2 metabolites were measured using competitive enzyme immunoassays (EIAs).
Our results indicate that COX-1 mRNA expression is significantly higher (P < 0.05) in ovarian tumor samples compared to normal ovaries while there is no significant difference in expression of COX-2 between the samples. Immunohistochemistry results support this finding and show COX-1 expression only in tumor samples and COX-2 expression unchanged between normal ovary and tumor samples. PGE2 levels are significantly higher (P < 0.05) in tumor samples compared to normal ovaries, and there is no significant difference in PGE2 metabolite levels between the samples.
These findings may implicate COX-1 as a suitable target for the prevention or treatment of ovarian cancer.
我们的目的是确定环氧化酶1（COX-1）和环氧化酶2（COX-2）在母鸡卵巢肿瘤和正常卵巢中的mRNA和蛋白表达，母鸡是人类卵巢癌的一个优秀模型。还测定了前列腺素E2（PGE2）和PGE2代谢物的组织浓度。
从2至4岁单冠白来航母鸡的卵巢肿瘤（n = 18）和正常卵巢（n = 29）获取组织。使用Sybr Green进行定量实时PCR以定量COX-1和COX-2的mRNA表达，以18S表达作为COX标准化的内对照。使用针对COX-1和COX-2的抗体进行免疫组织化学，以定位肿瘤（n = 5）和正常样本（n = 6）子集中每种同工型的蛋白表达。为了测定组织前列腺素浓度，从卵巢肿瘤（n = 8）和正常卵巢（n = 8）获取组织。使用竞争性酶免疫测定（EIA）测量PGE2和PGE2代谢物。
我们的结果表明，与正常卵巢相比，卵巢肿瘤样本中COX-1 mRNA表达显著更高（P < 0.05），而样本之间COX-2表达无显著差异。免疫组织化学结果支持这一发现，并显示仅在肿瘤样本中有COX-1表达，正常卵巢和肿瘤样本之间COX-2表达无变化。与正常卵巢相比，肿瘤样本中PGE2水平显著更高（P < 0.05），样本之间PGE2代谢物水平无显著差异。
这些发现可能表明COX-1是预防或治疗卵巢癌的合适靶点。
