Kotay Shireen Meher, Das Debabrata
Fermentation Technology Laboratory, Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.
Bioresour Technol. 2007 Apr;98(6):1183-90. doi: 10.1016/j.biortech.2006.05.009. Epub 2006 Jun 23.
Bacillus coagulans strain IIT-BT S1 isolated from anaerobically digested activated sewage sludge was investigated for its ability to produce H(2) from glucose-based medium under the influence of different environmental parameters. At mid-exponential phase of cell growth, H(2) production initiated and reached maximum production rate in the stationary phase. The maximal H(2) yield (2.28 mol H(2)/molglucose) was recorded at an initial glucose concentration of 2% (w/v), pH 6.5, temperature 37 degrees C, inoculum volume of 10% (v/v) and inoculum age of 14 h. Cell growth rate and rate of hydrogen production decreased when glucose concentration was elevated above 2% w/v, indicating substrate inhibition. The ability of the organism to utilize various carbon sources for H(2) fermentation was also determined.
对从厌氧消化活性污水污泥中分离出的凝结芽孢杆菌菌株IIT-BT S1，研究了其在不同环境参数影响下从基于葡萄糖的培养基中产生氢气的能力。在细胞生长的指数中期阶段，氢气产生开始，并在稳定期达到最大产率。在初始葡萄糖浓度为2%（w/v）、pH值6.5、温度37摄氏度、接种量10%（v/v）和接种龄14小时的条件下，记录到最大氢气产量（2.28摩尔氢气/摩尔葡萄糖）。当葡萄糖浓度升高到2% w/v以上时，细胞生长速率和氢气产生速率下降，表明存在底物抑制。还测定了该生物体利用各种碳源进行氢气发酵的能力。
