Massanet-Nicolau Jaime, Dinsdale Richard, Guwy Alan
Faculty of Health, Sport and Science, University of Glamorgan, Pontypridd, CF37 1DL, UK.
Bioresour Technol. 2008 Sep;99(14):6325-31. doi: 10.1016/j.biortech.2007.12.012. Epub 2008 Jan 28.
Hydrogen was successfully produced by fermenting primary sewage sludge which had been both heat treated and digested with a commercially available enzyme preparation. When either heat treatment or enzymatic digestion were not used, no hydrogen was produced during fermentation. Heat treated mesophilic anaerobic sludge was used as an inoculum rather than a pure microbial culture. Fermentation was conducted at pH levels ranging from of 4.5 to 7.0. When fermentation took place at pH 5.5 a peak hydrogen production rate of 3.75 ml min(-1) was observed. At this pH the hydrogen yield was 0.37 mol H(2)mol(-1) carbohydrate, equivalent to 18.14L H(2)kg(-1) dry solids.
通过利用市售酶制剂对经过热处理和消化处理的原生污水污泥进行发酵，成功产生了氢气。当未进行热处理或酶消化时，发酵过程中未产生氢气。使用经过热处理的中温厌氧污泥作为接种物，而非纯微生物培养物。发酵在pH值为4.5至7.0的范围内进行。当在pH 5.5进行发酵时，观察到氢气产生速率峰值为3.75 ml min(-1)。在此pH值下，氢气产率为0.37 mol H(2)mol(-1)碳水化合物，相当于18.14L H(2)kg(-1)干固体。
