Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet, Kongens Lyngby, Denmark.
J Appl Microbiol. 2009 Mar;106(3):1041-9. doi: 10.1111/j.1365-2672.2008.04071.x. Epub 2009 Jan 30.
The aim of this study was to enrich, characterize and identify strict anaerobic extreme thermophilic hydrogen (H(2)) producers from digested household solid wastes.
A strict anaerobic extreme thermophilic H(2) producing bacterial culture was enriched from a lab-scale digester treating household wastes at 70 degrees C. The enriched mixed culture consisted of two rod-shaped bacterial members growing at an optimal temperature of 80 degrees C and an optimal pH 8.1. The culture was able to utilize glucose, galactose, mannose, xylose, arabinose, maltose, sucrose, pyruvate and glycerol as carbon sources. Growth on glucose produced acetate, H(2) and carbon dioxide. Maximal H(2) production rate on glucose was 1.1 mmol l(-1) h(-1) with a maximum H(2) yield of 1.9 mole H(2) per mole glucose. 16S ribosomal DNA clone library analyses showed that the culture members were phylogenetically affiliated to the genera Bacillus and Clostridium. Relative abundance of the culture members, assessed by fluorescence in situ hybridization, were 87 +/- 5% and 13 +/- 5% for Bacillus and Clostridium, respectively.
An extreme thermophilic, strict anaerobic, mixed microbial culture with H(2)-producing potential was enriched from digested household wastes.
This study provided a culture with a potential to be applied in reactor systems for extreme thermophilic H(2) production from complex organic wastes.
本研究旨在从消化家庭固体废物中富集、表征和鉴定严格厌氧极端嗜热氢气（H(2)）产生菌。
从处理家庭废物的实验室规模消化器中，从 70°C 下富集了严格厌氧极端嗜热 H(2)产生细菌培养物。富集的混合培养物由两个棒状细菌成员组成，它们在 80°C 的最佳温度和 8.1 的最佳 pH 值下生长。该培养物能够利用葡萄糖、半乳糖、甘露糖、木糖、阿拉伯糖、麦芽糖、蔗糖、丙酮酸和甘油作为碳源。在葡萄糖上的生长产生乙酸、H(2)和二氧化碳。在葡萄糖上最大的 H(2)产生速率为 1.1 mmol l(-1) h(-1)，最大的 H(2)产率为 1.9 摩尔 H(2)每摩尔葡萄糖。16S 核糖体 DNA 克隆文库分析表明，培养物成员与芽孢杆菌属和梭菌属密切相关。通过荧光原位杂交评估的培养物成员的相对丰度分别为 87 +/- 5%和 13 +/- 5%，分别为芽孢杆菌和梭菌。
从消化的家庭废物中富集了具有产生 H(2)潜力的极端嗜热、严格厌氧、混合微生物培养物。
本研究提供了一种具有从复杂有机废物中产生极端嗜热 H(2)的潜力的培养物。
