State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Academy of Sciences, Nanning, China.
College of Life Science and Technology, Guangxi University, Nanning, China.
Front Cell Infect Microbiol. 2021 Dec 15;11:777266. doi: 10.3389/fcimb.2021.777266. eCollection 2021.
is one of the important human and plant pathogens causing not only invasive aspergillosis in immunocompromised patients but also crop contamination resulting from carcinogenic aflatoxins (AFs). Investigation of the targeting factors that are involved in pathogenicity is of unmet need to dismiss the hazard. Phosphoglucose isomerase (PGI) catalyzes the reversible conversion between glucose-6-phosphate and fructose-6-phosphate, thus acting as a key node for glycolysis, pentose phosphate pathway, and cell wall biosynthesis in fungi. In this study, we constructed an deletion mutant, which exhibited specific carbon requirement for survival, reduced conidiation, and slowed germination even under optimal experimental conditions. The Δ mutant lost the ability to form sclerotium and displayed hypersusceptibility to osmotic, oxidative, and temperature stresses. Furthermore, significant attenuated virulence of the Δ mutant was documented in the infection model, larval model, and crop seeds. Our results indicate that PGI in is a key enzyme in maintaining sugar homeostasis, stress response, and pathogenicity of . Therefore, PGI is a potential target for controlling infection and AF contamination caused by .
是一种重要的人类和植物病原体，不仅会导致免疫功能低下患者的侵袭性曲霉菌病，还会导致农作物受到致癌黄曲霉毒素（AFs）的污染。调查参与致病性的靶向因子是消除危害的未满足需求。磷酸葡萄糖异构酶（PGI）催化葡萄糖-6-磷酸和果糖-6-磷酸之间的可逆转化，因此在真菌中充当糖酵解、戊糖磷酸途径和细胞壁生物合成的关键节点。在这项研究中，我们构建了一个Δ突变体，该突变体表现出特定的生存碳需求，减少了分生孢子的形成，并在最佳实验条件下减缓了萌发。Δ突变体丧失了形成菌核的能力，并对渗透、氧化和温度胁迫表现出超敏性。此外，Δ突变体在感染模型、幼虫模型和作物种子中的毒力明显减弱。我们的结果表明，中的 PGI 是维持糖稳态、应激反应和致病性的关键酶。因此，PGI 是控制感染和由引起的 AF 污染的潜在靶标。
