后生动物细胞分化过程中DNA复制起点使用的拓宽
Broadening of DNA replication origin usage during metazoan cell differentiation.
作者信息
Dazy Sébastien, Gandrillon Olivier, Hyrien Olivier, Prioleau Marie-Noëlle
机构信息
Centre de Génétique Moléculaire et Cellulaire, Université Claude Bernard-Lyon I, 16 rue Dubois, 69622 Villeurbanne Cedex, France.
出版信息
EMBO Rep. 2006 Aug;7(8):806-11. doi: 10.1038/sj.embor.7400736. Epub 2006 Jun 16.
Abstract
We have examined whether replication of the chicken beta-globin locus changes during differentiation of primary erythroid progenitors into erythrocytes. In undifferentiated progenitors, four principal initiation sites and a replication fork pausing region (RFP) were observed. Forty-eight hours after induction of differentiation, the principal sites were maintained, even in the activated beta(A)-globin gene, some minor sites were enhanced, three new sites appeared and the RFP disappeared. One of the activated origins showed increased histone H3 K9K14 diacetylation, but the others did not. These results demonstrate a broadening of DNA replication origin usage during differentiation of untransformed metazoan cells and indicate that histone H3 diacetylation, other histone modifications so far reported and transcription are not crucial determinants of origin selection in this system.
摘要
我们研究了鸡β-珠蛋白基因座的复制在原代红系祖细胞分化为红细胞的过程中是否发生变化。在未分化的祖细胞中，观察到四个主要起始位点和一个复制叉暂停区域（RFP）。诱导分化48小时后，主要位点得以保留，即使在活化的β(A)-珠蛋白基因中也是如此，一些次要位点增强，出现了三个新位点，且RFP消失。其中一个活化的起始位点显示组蛋白H3 K9K14二乙酰化增加，但其他位点没有。这些结果表明，在未转化的后生动物细胞分化过程中，DNA复制起始位点的使用范围扩大，并且表明组蛋白H3二乙酰化、迄今报道的其他组蛋白修饰以及转录并非该系统中起始位点选择的关键决定因素。
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