Drysdale T A, Elinson R P
Department of Zoology, University of Toronto, Ontario, Canada.
Development. 1991 Feb;111(2):469-78. doi: 10.1242/dev.111.2.469.
An antibody that recognizes tyrosine hydroxylase can be used as a marker for hatching gland cells in Xenopus embryos. Using this marker, we have shown that hatching gland cells are induced at the end of gastrulation and that presumptive hatching gland cells are localized to the anterior neural folds in Xenopus. The movements of neurulation bring the hatching gland cells together to form a characteristic Y pattern on the dorsoanterior surface of the head. The Y pattern delineates several zones of surface ectoderm which can be visualized by the presence or absence of ciliated cells. As development proceeds the hatching gland pattern is altered, demonstrating the active changes involved in forming the face. Lithium, UV irradiation and retinoic acid can be used to alter the hatching gland pattern in specific ways which help to understand the underlying mechanisms of ectodermal patterning.
一种识别酪氨酸羟化酶的抗体可作为非洲爪蟾胚胎孵化腺细胞的标记物。利用这一标记物，我们已表明孵化腺细胞在原肠胚形成末期被诱导产生，且非洲爪蟾中推定的孵化腺细胞定位于前神经褶。神经胚形成的运动将孵化腺细胞聚集在一起，在头部背前表面形成特征性的Y形图案。Y形图案勾勒出几个表面外胚层区域，这些区域可通过纤毛细胞的有无来可视化。随着发育的进行，孵化腺图案会发生改变，这表明在面部形成过程中涉及到活跃的变化。锂、紫外线照射和视黄酸可用于以特定方式改变孵化腺图案，这有助于理解外胚层图案形成的潜在机制。
