Peelman L J, Van de Weghe A R, Coppieters W R, Van Zeveren A J, Bouquet Y H
Department of Animal Genetics and Breeding, Faculty of Veterinary Medicine, State University of Ghent (RUG), Merelbeke, Belgium.
Immunogenetics. 1991;34(3):192-5. doi: 10.1007/BF00205823.
A genomic clone, SSBf1, containing the complement factor B (BF), a major histocompatibility class III antigen, has been isolated from a porcine genomic library. Partial sequencing and comparison with a human BF gene has identified seven exons coding for amino acids of Ba and Bb, the two subunits of BF. The protein sequence similarity with the human BF is on the average 87%. Southern blot analysis confirmed the existence of only one BF gene per haploid genome. Restriction fragment length polymorphism typing with Taq I showed that there are at least three different porcine BF-haplotypes.
从猪基因组文库中分离出了一个基因组克隆体SSBf1，它包含主要组织相容性复合体III类抗原补体因子B（BF）。通过部分测序并与人BF基因进行比较，已确定了编码BF两个亚基Ba和Bb氨基酸的7个外显子。与人类BF的蛋白质序列相似性平均为87%。Southern印迹分析证实单倍体基因组中仅存在一个BF基因。用Taq I进行的限制性片段长度多态性分型表明，至少存在三种不同的猪BF单倍型。
