Zaffaroni M, Gallo L, Ghezzi A, Cazzullo C L
Centro Studi Sclerosi Multipla, University of Milan, S. Antonio Abate Hospital, Gallarate, Italy.
J Neurol. 1991 Jul;238(4):209-11. doi: 10.1007/BF00314782.
We studied paired cerebrospinal fluid (CSF) and peripheral blood (PB) samples from 18 inactive multiple sclerosis (MS) patients and 10 with non-inflammatory neurological diseases. By means of a dual-colour cytofluorimetric micromethod we were able to count 1500 cells on average in each CSF sample. We found a significant reduction of CD45RA+ and CD4+CD45RA+ cells in the CSF of MS patients. Similarly, CD45RA+ and CD4+CD45RA+ CSF/PB ratios were lower compared with controls. The reduction of suppressor-inducer T-cells did not correlate with CD8+ cell levels in the CSF. The CD4+ subset ratio (CD4+CD45RA-/CD4+CD45RA+) was significantly increased in the CSF of MS patients. Our data suggest that the reduction of CD4+CD45RA+ cells in the PB is not due to a segregation of such cells in the CSF. Conversely, CSF changes reflect changes in the PB similar to these found for other T-cell subsets.
我们研究了18例静止期多发性硬化症（MS）患者和10例非炎性神经系统疾病患者的配对脑脊液（CSF）和外周血（PB）样本。通过双色细胞荧光微方法，我们平均能够在每个脑脊液样本中计数1500个细胞。我们发现MS患者脑脊液中CD45RA+和CD4+CD45RA+细胞显著减少。同样，与对照组相比，脑脊液/外周血中CD45RA+和CD4+CD45RA+的比例更低。抑制诱导性T细胞的减少与脑脊液中CD8+细胞水平无关。MS患者脑脊液中CD4+亚群比例（CD4+CD45RA-/CD4+CD45RA+）显著增加。我们的数据表明，外周血中CD4+CD45RA+细胞的减少并非由于此类细胞在脑脊液中的隔离。相反，脑脊液的变化反映了外周血中的变化，类似于在其他T细胞亚群中发现的变化。
