Sundaram Kumaran, Panneerselvam Kavin S
Department of Medical Biochemistry, Dr. AL Mudaliar Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, 600 113, India.
Biogerontology. 2006 Apr;7(2):111-8. doi: 10.1007/s10522-006-0002-2.
The exposure of biological system to various conditions of oxidative stress is the major contributor for aging process. Oxidative stress in turn increases the cellular levels of oxidatively modified proteins, lipids and nucleic acids resulting in a loss of physical activity and metabolic integrity. In this study, we evaluated the role of L-carnitine and DL-alpha-lipoic acid in minimizing oxidant generation and macromolecular damage in skeletal muscle of aged rats. We found that the oxidant generation was increased in aged rat skeletal muscle when compared to young rats. There was a simultaneous increase in the levels of lipid peroxidation, protein carbonyl content and DNA strand breaks in aged rat skeletal muscle. Administration of L-carnitine (300 mg/kg body wt/day) and DL-alpha-lipoic acid (100 mg/kg body wt/day) to aged rats for 30 days, decreased the oxidant generation, lipid peroxidation, protein carbonylation and DNA strand breaks. We concluded that co-administration of carnitine and lipoic acid to aged rats has the potential to prevent oxidative stress mediated macromolecular damage in skeletal muscle of aged rats by their putative role as efficient antioxidants.
生物系统暴露于各种氧化应激条件下是衰老过程的主要促成因素。氧化应激反过来又会增加细胞内氧化修饰的蛋白质、脂质和核酸的水平，导致身体活动能力和代谢完整性的丧失。在本研究中，我们评估了左旋肉碱和硫辛酸在减少老年大鼠骨骼肌中氧化剂生成和大分子损伤方面的作用。我们发现，与年轻大鼠相比，老年大鼠骨骼肌中的氧化剂生成增加。老年大鼠骨骼肌中脂质过氧化水平、蛋白质羰基含量和DNA链断裂同时增加。给老年大鼠连续30天给予左旋肉碱（300毫克/千克体重/天）和硫辛酸（100毫克/千克体重/天），可降低氧化剂生成、脂质过氧化、蛋白质羰基化和DNA链断裂。我们得出结论，给老年大鼠同时给予肉碱和硫辛酸，有可能通过其作为有效抗氧化剂的假定作用，预防氧化应激介导的老年大鼠骨骼肌大分子损伤。
