Sander Ingrid, Raulf-Heimsoth Monika, Wiemer Kai, Kespohl Sabine, Brüning Thomas, Merget Rolf
Berufsgenossenschaftliches Forschungsinstitut für Arbeitsmedizin, Ruhr-Universität Bochum, Bochum, Deutschland.
Int Arch Allergy Immunol. 2006;141(1):51-6. doi: 10.1159/000094182. Epub 2006 Jun 27.
A pharmaceutical industry worker was exposed to dust of gum arabic in the tablet coating plant and complained of work-related shortness of breath, chest tightness, runny nose, itching and redness of the eyes. This case was investigated for allergy to gum arabic and compared with a control group. The aim of the study was to identify the IgE-binding components responsible for the work-related symptoms.
Skin prick tests (SPTs)and specific IgE (sIgE) measurements with environmental and occupational allergens, spirometry and a specific bronchial challenge with gum arabic were performed. One hundred and nineteen control subjects underwent SPT with gum arabic and 43 controls were tested for sIgE. Crossreactivity between gum arabic and horse radish peroxidase was investigated by IgE CAP inhibition. A combined procedure of immunoblotting and periodate treatment was applied to identify the epitope nature of gum arabic.
Allergy to gum arabic was shown by SPT, presence of sIgE and a positive bronchial challenge with gum arabic. Sensitization to gum arabic was demonstrated by SPT or sIgE in 7 and 5 controls, respectively. The results of inhibition with horse radish peroxidase, immunoblotting and periodate treatment suggest that gum arabic sIgE of the patient and 1 SPT-positive control subject were directed to the polypeptide chains of gum arabic. In contrast, gum arabic sIgE of the other controls reacted to carbohydrate components.
Sensitization to gum arabic carbohydrate structures occurs casually in atopic patients with pollen sensitization without obvious exposure to gum arabic. This study suggests that allergy to gum arabic is mediated preferentially by IgE antibodies directed to polypeptide chains of gum arabic.
一名制药行业工人在片剂包衣车间接触了阿拉伯树胶粉尘，出现了与工作相关的呼吸急促、胸闷、流鼻涕、眼睛瘙痒和发红等症状。对该病例进行了阿拉伯树胶过敏调查，并与对照组进行了比较。本研究的目的是确定导致这些与工作相关症状的IgE结合成分。
进行了皮肤点刺试验（SPT）、针对环境和职业过敏原的特异性IgE（sIgE）检测、肺功能测定以及用阿拉伯树胶进行的特异性支气管激发试验。119名对照受试者接受了阿拉伯树胶的SPT检测，43名对照受试者进行了sIgE检测。通过IgE CAP抑制试验研究了阿拉伯树胶与辣根过氧化物酶之间的交叉反应性。采用免疫印迹和高碘酸盐处理相结合的方法来确定阿拉伯树胶的表位性质。
SPT、sIgE的存在以及阿拉伯树胶支气管激发试验阳性均表明对阿拉伯树胶过敏。分别有7名和5名对照受试者通过SPT或sIgE检测显示对阿拉伯树胶致敏。辣根过氧化物酶抑制试验、免疫印迹和高碘酸盐处理的结果表明，患者和1名SPT阳性对照受试者的阿拉伯树胶sIgE针对阿拉伯树胶的多肽链。相比之下，其他对照受试者的阿拉伯树胶sIgE对碳水化合物成分有反应。
在对花粉致敏的特应性患者中，即使没有明显接触阿拉伯树胶，也可能偶然发生对阿拉伯树胶碳水化合物结构的致敏。本研究表明，对阿拉伯树胶的过敏主要由针对阿拉伯树胶多肽链的IgE抗体介导。
