[乙肝表面抗原阴性肝病患者肝内乙肝病毒DNA的检测]

[Detection of Intrahepatic HBV DNA in HBsAg-negative liver diseases].

作者信息

Kim Yun Soo, Jang Jae Young, Eun Soo Hoon, Cheon Young Koog, Kim Young Seok, Moon Jong Ho, Cho Young Deok, Jin So-Young, Shim Chan Sup, Kim Boo Sung

机构信息

Department of Gastroenterology, Gil Medical Center, Gachon University, Inchon, Korea.

出版信息

Korean J Hepatol. 2006 Jun;12(2):201-8.

PMID:16804345

Abstract

BACKGROUNDS/AIMS: Occult HBV infection is characterized by the presence of HBV infection with undetectable HBsAg. This study was carried out to find out the frequency of HBV infection in HBsAg- negative patients.

METHODS

Fifty-six HBsAg-negative patients including 17 anti-HCV positive patients were evaluated. Patients were grouped according to their serological status; group A (anti-HBc+, anti-HBs-, n=16), B (anti-HBc+, anti-HBs+, n=26), and C (anti-HBc-, anti-HBs+/-, n=14). DNA was extracted from frozen liver biopsy specimen, and HBV DNA level was measured with real-time PCR.

RESULTS

Overall frequency of detectable intrahepatic HBV DNA was 34% (19/56). The frequency was 56% (9/16) in group A, 31% (8/26) in group B and 14% (2/14) in group C (P=0.01). Intrahepatic HBV DNA levels were as follows; 2,010 +/- 6,660 copies/mg in group A, 6,180 +/- 29,530 copies/mg in group B and 350 +/- 1,220 copies/mg in group C. The frequency of occult HBV infection was not increased in anti-HCV positive patients.

CONCLUSIONS

Intrahepatic HBV DNA is frequently detected in anti-HBc positive, HBsAg-negative patients, although the concentration is low.

摘要

背景/目的：隐匿性HBV感染的特征是存在HBV感染但HBsAg检测不到。本研究旨在查明HBsAg阴性患者中HBV感染的频率。

方法

对56例HBsAg阴性患者进行评估，其中包括17例抗-HCV阳性患者。根据血清学状态将患者分组；A组（抗-HBc阳性，抗-HBs阴性，n = 16），B组（抗-HBc阳性，抗-HBs阳性，n = 26），C组（抗-HBc阴性，抗-HBs阳性或阴性，n = 14）。从冷冻的肝活检标本中提取DNA，并用实时PCR测量HBV DNA水平。

结果

肝内可检测到HBV DNA的总体频率为34%（19/56）。A组频率为56%（9/16），B组为31%（8/26），C组为14%（2/14）（P = 0.01）。肝内HBV DNA水平如下：A组为2,010±6,660拷贝/毫克，B组为6,180±29,530拷贝/毫克，C组为350±1,220拷贝/毫克。抗-HCV阳性患者中隐匿性HBV感染的频率没有增加。