Kim Yun Soo, Jang Jae Young, Eun Soo Hoon, Cheon Young Koog, Kim Young Seok, Moon Jong Ho, Cho Young Deok, Jin So-Young, Shim Chan Sup, Kim Boo Sung
Department of Gastroenterology, Gil Medical Center, Gachon University, Inchon, Korea.
Korean J Hepatol. 2006 Jun;12(2):201-8.
BACKGROUNDS/AIMS: Occult HBV infection is characterized by the presence of HBV infection with undetectable HBsAg. This study was carried out to find out the frequency of HBV infection in HBsAg- negative patients.
Fifty-six HBsAg-negative patients including 17 anti-HCV positive patients were evaluated. Patients were grouped according to their serological status; group A (anti-HBc+, anti-HBs-, n=16), B (anti-HBc+, anti-HBs+, n=26), and C (anti-HBc-, anti-HBs+/-, n=14). DNA was extracted from frozen liver biopsy specimen, and HBV DNA level was measured with real-time PCR.
Overall frequency of detectable intrahepatic HBV DNA was 34% (19/56). The frequency was 56% (9/16) in group A, 31% (8/26) in group B and 14% (2/14) in group C (P=0.01). Intrahepatic HBV DNA levels were as follows; 2,010 +/- 6,660 copies/mg in group A, 6,180 +/- 29,530 copies/mg in group B and 350 +/- 1,220 copies/mg in group C. The frequency of occult HBV infection was not increased in anti-HCV positive patients.
Intrahepatic HBV DNA is frequently detected in anti-HBc positive, HBsAg-negative patients, although the concentration is low.
背景/目的:隐匿性HBV感染的特征是存在HBV感染但HBsAg检测不到。本研究旨在查明HBsAg阴性患者中HBV感染的频率。
对56例HBsAg阴性患者进行评估,其中包括17例抗-HCV阳性患者。根据血清学状态将患者分组;A组(抗-HBc阳性,抗-HBs阴性,n = 16),B组(抗-HBc阳性,抗-HBs阳性,n = 26),C组(抗-HBc阴性,抗-HBs阳性或阴性,n = 14)。从冷冻的肝活检标本中提取DNA,并用实时PCR测量HBV DNA水平。
肝内可检测到HBV DNA的总体频率为34%(19/56)。A组频率为56%(9/16),B组为31%(8/26),C组为14%(2/14)(P = 0.01)。肝内HBV DNA水平如下:A组为2,010±6,660拷贝/毫克,B组为6,180±29,530拷贝/毫克,C组为350±1,220拷贝/毫克。抗-HCV阳性患者中隐匿性HBV感染的频率没有增加。
在抗-HBc阳性、HBsAg阴性患者中经常检测到肝内HBV DNA,尽管浓度较低。