Mas Mehmet Refik, Isik Ahmet Turan, Yamanel Levent, Inal Volkan, Tasci Ilker, Deveci Salih, Mas Nuket, Comert Bilgin, Akay Cemal
Department of Internal Medicine, Gulhane School of Medicine, Ankara, Turkey.
Pancreas. 2006 Jul;33(1):77-81. doi: 10.1097/01.mpa.0000222316.74607.07.
Based on the results of recent studies that reported depleted antioxidant capacity in patients with chronic pancreatitis (CP), prevention of free radical production has gained importance in antifibrotic treatment strategies in CP. The aim of this study was to investigate the effects of taurine on oxidative capacity and fibrosis in experimental chronic rat pancreatic fibrosis.
CP was induced in male Sprague-Dawley rats by intraductal trinitrobenzene sulfonic acid (TNBS) dissolved in ethanol. Taurine was given intraperitoneally at a concentration of 1000 mg/kg. The treatment groups were as follows: group 1, TNBS plus normal saline (NS); group 2, TNBS plus taurine; group 3, ethanol plus NS; and group 4, NS plus NS. Each group contained 15 animals. Treatment was started after established CP. After 4 weeks of treatment, markers of oxidative stress and the degree of pancreatic fibrosis were determined.
The amount of weight loss was significantly lower in the taurine-treated group with CP (P < 0.002). Tissue malondialdehyde levels increased and superoxide dismutase and glutathione peroxidase activities decreased significantly after treatment as well (P < 0.001). Histopathologic scores were also lower in taurine-treated animals with CP (P < 0.005).
Taurine treatment improved the degree of oxidative stress and fibrosis in rat CP. Antioxidant treatment might be considered a novel option to alleviate the fibrotic process in CP.
基于近期研究报道慢性胰腺炎（CP）患者抗氧化能力下降，预防自由基产生在CP的抗纤维化治疗策略中变得愈发重要。本研究旨在探讨牛磺酸对实验性大鼠慢性胰腺纤维化氧化能力和纤维化的影响。
通过向雄性Sprague-Dawley大鼠胰管内注射溶解于乙醇的三硝基苯磺酸（TNBS）诱导CP。以1000 mg/kg的浓度腹腔注射牛磺酸。治疗组如下：第1组，TNBS加生理盐水（NS）；第2组，TNBS加牛磺酸；第3组，乙醇加NS；第4组，NS加NS。每组包含15只动物。在CP形成后开始治疗。治疗4周后，测定氧化应激标志物和胰腺纤维化程度。
CP牛磺酸治疗组体重减轻量显著更低（P < 0.002）。治疗后组织丙二醛水平升高，超氧化物歧化酶和谷胱甘肽过氧化物酶活性也显著降低（P < 0.001）。CP牛磺酸治疗动物的组织病理学评分也更低（P < 0.005）。
牛磺酸治疗改善了大鼠CP的氧化应激程度和纤维化。抗氧化治疗可能被认为是减轻CP纤维化进程的一种新选择。
