Department of Gastroenterology, Affiliated Changhai Hospital, 2nd Military Medical University, Shanghai 200433, People's Republic of China.
Pancreas. 2011 Oct;40(7):1091-6. doi: 10.1097/MPA.0b013e31821b59c6.
The α-tocopherol and tocotrienol-rich fraction (TRF) are considered effective antioxidants. This study aimed to compare the antioxidative and antifibrotic effects of α-tocopherol and TFR in dibutylin dichloride (DBTC)-induced chronic pancreatitis (CP) rats.
Oral administration of α-tocopherol and TFR (both 800 mg/kg per day) started the next day after DBTC (8 mg/kg) infusion into the tail vein for 4 weeks. Histological examination, Sirius red staining, and measurement of the contents of hydroxyproline and malondialdehyde of the pancreas were performed to evaluate pancreatic damage and fibrosis. Immunohistochemical analysis of α-smooth muscle actin and real-time reverse transcription polymerase chain reaction for transforming growth factor-β1 (TGF-β1) and collagen-α1(I) were performed to evaluate the activation of pancreatic stellate cells and the mRNA levels of fibrosis-related genes, respectively.
Both α-tocopherol and TRF reduced oxidative stress, ameliorated inflammation and fibrosis, and down-regulated the mRNA expression of TGF-β1 and collagen-α1(I) in DBTC-induced CP. The TRF was superior to α-tocopherol in alleviating inflammation and fibrosis and down-regulating TGF-β1 mRNA expression.
Oral administration of α-tocopherol and TRF improves pancreatic inflammation and fibrosis in DBTC-induced CP rats, with TRF being more effective than α-tocopherol. Therefore, TRF may be a novel option for alleviating inflammation and, particularly, the fibrotic process in CP.
α-生育酚和生育三烯酚富含组（TRF）被认为是有效的抗氧化剂。本研究旨在比较α-生育酚和 TFR 在二丁基二氯化锡（DBTC）诱导的慢性胰腺炎（CP）大鼠中的抗氧化和抗纤维化作用。
DBTC（8mg/kg）尾静脉输注后第二天开始给予α-生育酚和 TFR（每天 800mg/kg）口服治疗，共 4 周。进行组织学检查、天狼星红染色以及胰腺羟脯氨酸和丙二醛含量的测量，以评估胰腺损伤和纤维化。免疫组织化学分析α-平滑肌肌动蛋白和实时逆转录聚合酶链反应（RT-PCR）用于转化生长因子-β1（TGF-β1）和胶原-α1（I）的 mRNA 水平，分别评估胰腺星状细胞的激活和纤维化相关基因的 mRNA 水平。
α-生育酚和 TRF 均可减轻氧化应激，改善炎症和纤维化，并下调 DBTC 诱导的 CP 中 TGF-β1 和胶原-α1（I）的 mRNA 表达。与 α-生育酚相比，TRF 更能减轻炎症和纤维化，下调 TGF-β1 mRNA 表达。
口服α-生育酚和 TRF 可改善 DBTC 诱导的 CP 大鼠的胰腺炎症和纤维化，TRF 比 α-生育酚更有效。因此，TRF 可能是缓解 CP 中炎症和纤维化的一种新选择。
