Department of Geratology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Pancreatology. 2011;11(1):5-11. doi: 10.1159/000309252. Epub 2011 Feb 10.
To investigate the effects of α-tocopherol on pancreatic fibrosis and survival in rats with experimental chronic pancreatitis induced by trinitrobenzene sulfonic acid (TNBS).
Chronic pancreatitis was induced in male Sprague-Dawley rats by infusion of TNBS into the pancreatic duct. α-Tocopherol (300, 600 or 900 mg/kg) was orally administered to rats with experimental pancreatitis (treatment group) daily for 4 weeks. The relative pancreatic weight, pancreatic pseudocyst and death rate were observed. Paraffin-embedded tissue samples were sliced, stained by hematoxylin-eosin and histopathologically examined.
α-Tocopherol administration significantly ameliorated the pancreatic weight loss induced by TNBS in chronic pancreatitis rats compared to the control group. There were pancreatic pseudocysts in 69% of the α-tocopherol group, and in 100% of the control group. α-Tocopherol administration led to a significant increase of the survival rate. The histopathologic scores were higher in the control group than in the α-tocopherol group. Subgroup analysis of histopathologic scores revealed that a high dose of α-tocopherol results in less pancreatic injuries.
α-Tocopherol treatment elevates survival rate, extenuates fibrosis and increases relative pancreatic weight in the chronic pancreatitis model. α-Tocopherol therapy in chronic pancreatitis is now required to confirm these findings and establish the role of this treatment in the management of this disabling condition. and IAP.
研究 α-生育酚对三硝基苯磺酸（TNBS）诱导的实验性慢性胰腺炎大鼠胰腺纤维化和生存的影响。
通过向胰管内输注 TNBS 诱导雄性 Sprague-Dawley 大鼠发生慢性胰腺炎。将 α-生育酚（300、600 或 900mg/kg）每日给予实验性胰腺炎大鼠（治疗组）口服，持续 4 周。观察相对胰腺重量、胰腺假性囊肿和死亡率。对石蜡包埋组织样本进行切片、苏木精-伊红染色和组织病理学检查。
与对照组相比，α-生育酚给药显著改善了 TNBS 诱导的慢性胰腺炎大鼠的胰腺重量减轻。α-生育酚组有 69%发生胰腺假性囊肿，而对照组则有 100%发生。α-生育酚给药导致存活率显著增加。对照组的组织病理学评分高于α-生育酚组。组织病理学评分的亚组分析显示，高剂量的α-生育酚可导致较少的胰腺损伤。
α-生育酚治疗可提高慢性胰腺炎模型的生存率、减轻纤维化并增加相对胰腺重量。目前需要进行慢性胰腺炎的 α-生育酚治疗，以证实这些发现，并确定这种治疗在管理这种致残疾病中的作用。IAP。
