Sebaihia Mohammed, Wren Brendan W, Mullany Peter, Fairweather Neil F, Minton Nigel, Stabler Richard, Thomson Nicholas R, Roberts Adam P, Cerdeño-Tárraga Ana M, Wang Hongmei, Holden Matthew T G, Wright Anne, Churcher Carol, Quail Michael A, Baker Stephen, Bason Nathalie, Brooks Karen, Chillingworth Tracey, Cronin Ann, Davis Paul, Dowd Linda, Fraser Audrey, Feltwell Theresa, Hance Zahra, Holroyd Simon, Jagels Kay, Moule Sharon, Mungall Karen, Price Claire, Rabbinowitsch Ester, Sharp Sarah, Simmonds Mark, Stevens Kim, Unwin Louise, Whithead Sally, Dupuy Bruno, Dougan Gordon, Barrell Bart, Parkhill Julian
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.
Nat Genet. 2006 Jul;38(7):779-86. doi: 10.1038/ng1830. Epub 2006 Jun 25.
We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.
我们测定了艰难梭菌630菌株的全基因组序列，该菌株具有致病性且对多种药物耐药。我们的分析表明，基因组的很大一部分（11%）由可移动遗传元件组成，主要形式为接合转座子。这些可移动元件可能是艰难梭菌获取大量与抗菌耐药性、毒力、宿主相互作用及表面结构产生相关基因的原因。基因组中编码的代谢能力显示出在肠道环境中生存和生长的多种适应性。全基因组微阵列分析证实了基因组的极端变异性；这可能反映了该生物体在肠道中的生态位，并应为该生物体毒力的进化提供信息。
