Huang Keng-Shiang, Lai Tzung-Heng, Lin Yu-Cheng
Department of Engineering Science, National Cheng Kung University, 1 University Road, 701 Tainan, Taiwan.
Lab Chip. 2006 Jul;6(7):954-7. doi: 10.1039/b606424h. Epub 2006 May 18.
In this paper the manipulation of Ca-alginate microspheres, using a microfluidic chip, for the encapsulation of gold nanoparticles is presented. Our strategy is based on hydrodynamic-focusing on the forming of a series of self-assembling sphere structures, the so-called water-in-oil (w/o) emulsions, in the cross-junction microchannel. These fine emulsions, consisting of aqueous Na-alginates, are then dripped into a solution of 20% calcium salt to accomplish Ca-alginate microspheres in an efficient manner. Experimental data show that microspheres with diameters ranging from 50 microm to 2000 microm with a variation less than 5% were precisely generated. The size and gap of the droplets are tunable by adjusting the relative sheath/sample flow rate ratio. Furthermore, we applied them to encapsulated gold nanoparticles, and this one shot operation performs the 'Lab on a Chip'.
本文介绍了利用微流控芯片操控海藻酸钙微球以封装金纳米颗粒的方法。我们的策略基于在交叉微通道中通过流体动力聚焦形成一系列自组装球形结构，即所谓的油包水（w/o）乳液。然后将这些由海藻酸钠水溶液组成的精细乳液滴入20%钙盐溶液中，高效地制成海藻酸钙微球。实验数据表明，精确生成了直径范围为50微米至2000微米且变化小于5%的微球。通过调节相对鞘流/样品流速比，液滴的大小和间距是可调的。此外，我们将其应用于封装金纳米颗粒，这一一次性操作实现了“芯片实验室”。
