重组人生长激素对裸鼠人胃癌移植瘤模型生长的影响。
Effects of recombinant human growth hormone on growth of human gastric carcinoma xenograft model in nude mice.
作者信息
Liang Dao-Ming, Chen Jia-Yong, Zhang Yi, Gan Ping, Lin Jie, Chen An-Bao
机构信息
Department of Emergency Surgery of Second Affiliated Hospital, Kunming Medical College, Yunnan Province, China.
出版信息
World J Gastroenterol. 2006 Jun 28;12(24):3810-3. doi: 10.3748/wjg.v12.i24.3810.
AIM
To study effects of recombinant human growth hormone (rhGH) on growth of a human gastric carcinoma cell in vivo.
METHODS
Experimental mice were divided into control group, rhGH group, oxaliplatin (L-OHP) group and rhGH+L-OHP group. Cultured human gastric carcinoma cells BGC823 were inoculated into right axilla of nude mice and carcinoma xenograft model was established successfully. Inhibitory rate of xenograft tumor growth was estimated by measuring tumor volume; expression of proliferating cell nuclear antigen (PCNA), Bax and Bcl-2 proteins of xenograft tumor was detected using immunohistochemical S-P method.
RESULTS
Tumor growth inhibitory rate, the positive expression rate of PCNA, Bax and Bcl-2 were 49.3%, 58.2%, 65.2% and 59.2% in rhGH+L-OHP group respectively; 46.6%, 62.5%, 59.7% and 64.7% in L-OHP group; 5.0%, 82.7%, 23.2% and 82.2% in rhGH group and 0, 77.8%, 23.5% and 80.3% in control group. There was significant difference between rhGH+L-OHP group (or L-OHP group ) and control group or rhGH group (P < 0.05), whereas there were no significant differences (P > 0.05) between L-OHP group and rhGH+L-OHP group and between rhGH group and control group.
CONCLUSION
rhGH does not accelerate the proli-feration of human gastric cancer cell in vivo.
目的
研究重组人生长激素（rhGH）对人胃癌细胞体内生长的影响。
方法
将实验小鼠分为对照组、rhGH组、奥沙利铂（L-OHP）组和rhGH+L-OHP组。将培养的人胃癌细胞BGC823接种于裸鼠右腋部，成功建立癌异种移植模型。通过测量肿瘤体积评估异种移植瘤生长抑制率；采用免疫组织化学S-P法检测异种移植瘤增殖细胞核抗原（PCNA）、Bax和Bcl-2蛋白的表达。
结果
rhGH+L-OHP组肿瘤生长抑制率、PCNA、Bax和Bcl-2阳性表达率分别为49.3%、58.2%、65.2%和59.2%；L-OHP组分别为46.6%、62.5%、59.7%和64.7%；rhGH组分别为5.0%、82.7%、23.2%和82.2%；对照组分别为0、77.8%、23.5%和80.3%。rhGH+L-OHP组（或L-OHP组）与对照组或rhGH组之间差异有统计学意义（P<0.05），而L-OHP组与rhGH+L-OHP组之间以及rhGH组与对照组之间差异无统计学意义（P>0.05）。
结论
rhGH在体内不会促进人胃癌细胞的增殖。
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