重组人生长激素对人胃癌细胞系BGC823细胞生长的体外作用
In vitro effects of recombinant human growth hormone on growth of human gastric cancer cell line BGC823 cells.
作者信息
Chen Jia-Yong, Liang Dao-Ming, Gan Ping, Zhang Yi, Lin Jie
机构信息
Department of General Surgery of the Second Affiliated Hospital, Kunming Medical College, Kunming 650101, Yunnan Province, China. chenjiayong776@.hotmail.com
出版信息
World J Gastroenterol. 2004 Apr 15;10(8):1132-6. doi: 10.3748/wjg.v10.i8.1132.
AIM
To study the effects of recombinant human growth hormone (rhGH) on growth of human gastric cancer cell line in vitro.
METHODS
Experiment was divided into control group, rhGH group, oxaliplatin (L-OHP) group and rhGH+L-OHP group. Cell inhibitory rate, cell cycle, cell proliferation index (PI) and DNA inhibitory rate of human gastric cancer line BGC823, at different concentrations of rhGH treatment were studied by cell culture, MTT assay and flow cytometry.
RESULTS
The distinctly accelerated effects of rhGH on multiplication of BGC823 cell line were not found in vitro. There was no statistical significance between rhGH group and control group, or between rhGH+L-OHP group and L-OHP group (P>0.05). The cell growth curve did not rise. Cell inhibitory rate and cells arrested in G(0)-G(1) phase were obviously increased. Meanwhile, cells in S phase and PI were distinctly decreased and DNA inhibitory rate was obviously increased in rhGH+L-OHP group in comparison with control group and rhGH group, respectively (P<0.01). Cell inhibitory rate showed an increasing trend and PI showed a decreasing trend in rhGH+L-OHP group compared with L-OHP group.
CONCLUSION
In vitro rhGH does not accelerate the multiplication of human gastric cancer cells. It may increase the therapeutic efficacy when it is used in combination with anticancer drugs.
目的
研究重组人生长激素（rhGH）对人胃癌细胞系体外生长的影响。
方法
实验分为对照组、rhGH组、奥沙利铂（L-OHP）组和rhGH+L-OHP组。采用细胞培养、MTT法和流式细胞术研究不同浓度rhGH处理下人胃癌细胞系BGC823的细胞抑制率、细胞周期、细胞增殖指数（PI）和DNA抑制率。
结果
体外未发现rhGH对BGC823细胞系增殖有明显的促进作用。rhGH组与对照组之间，以及rhGH+L-OHP组与L-OHP组之间差异均无统计学意义（P>0.05）。细胞生长曲线未上升。细胞抑制率及停滞于G(0)-G(1)期的细胞明显增多。同时，与对照组和rhGH组相比，rhGH+L-OHP组S期细胞和PI明显降低，DNA抑制率明显升高（P<0.01）。与L-OHP组相比，rhGH+L-OHP组细胞抑制率呈上升趋势，PI呈下降趋势。
结论
体外rhGH不能促进人胃癌细胞增殖。与抗癌药物联合使用时可能提高治疗效果。
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