Surowiak Paweł, Suchocki Sławomir, Györffy Balazs, Gansukh Tserenchunt, Wojnar Andrzej, Maciejczyk Adam, Pudełko Marek, Zabel Maciej
Department of Histology and Embryology, University School of Medicine, Wrocław, Poland.
Folia Histochem Cytobiol. 2006;44(2):111-6.
It is suggested that tumour stromal myofibroblasts exert an unfavourable effect on the biology of breast cancer. We are aware of only a single study which examined relationships between manifestation of myofibroblasts in the stroma of breast cancer and clinicopathological data of the patients. The present study was aimed at estimation of the effect exerted by myofibroblasts present in the tumour stroma on principal pathological parameters and on expression of Ki67, P53 and HER-2 proteins in the group of the most frequent breast cancers, the ductal cancers. In paraffin sections of 60 ductal breast cancers (20 cases in G1, 20 in G2 and 20 in G3), immunohistochemical reactions were performed to detect expression of smooth muscle actin (SMA) in order to visualize myofibroblasts, Ki67, P53 and HER-2. The studies demonstrated that the most numerous myofibroblasts were present in G3 cases and they were the least frequent in G1 cases (P = 0.02). Positive correlations were observed between the presence of myofibroblasts in tumour stroma and expression of Ki67 and HER-2 in breast cancer cells in the entire group (P < 0.001 and P = 0.001, respectively), in G2 cases (P = 0.003 and P = 0.03) and in G3 cases (P = 0.01 and P = 0.03). Considering that the higher grade, Ki67 and HER-2 are thought to represent unfavourable prognostic factors, the elevated content of myofibroblasts in tumour stroma is probably typical for cases with worse prognosis.
有研究表明，肿瘤基质中的肌成纤维细胞对乳腺癌生物学特性具有不利影响。据我们所知，仅有一项研究探讨了乳腺癌基质中肌成纤维细胞的表现与患者临床病理数据之间的关系。本研究旨在评估肿瘤基质中存在的肌成纤维细胞对最常见的乳腺癌类型——导管癌的主要病理参数以及Ki67、P53和HER-2蛋白表达的影响。在60例乳腺导管癌的石蜡切片（G1组20例、G2组20例、G3组20例）中，进行免疫组织化学反应以检测平滑肌肌动蛋白（SMA）的表达，从而显示肌成纤维细胞、Ki67、P53和HER-2。研究表明，G3组病例中肌成纤维细胞数量最多，G1组病例中最少（P = 0.02）。在整个研究组中（分别为P < 0.001和P = 0.001）、G2组病例中（P = 0.003和P = 0.03）以及G3组病例中（P = 0.01和P = 0.03），均观察到肿瘤基质中肌成纤维细胞的存在与乳腺癌细胞中Ki67和HER-2的表达呈正相关。鉴于较高的分级、Ki67和HER-2被认为是不良预后因素，肿瘤基质中肌成纤维细胞含量升高可能是预后较差病例的典型特征。
