Zhong Lai-Ping, Zhao Shi-Fang, Chen Guan-Fu, Ping Fei-Yun, Xu Ze-Feng, Hu Ji-An
Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital, College of Medicine, Zhejiang University, No. 395 Yan'an Road, Hangzhou 310006, China.
Arch Oral Biol. 2006 Dec;51(12):1112-9. doi: 10.1016/j.archoralbio.2006.05.005. Epub 2006 Jun 27.
Oral squamous cell carcinoma (OSCC) is the most common malignant tumour in the oral and maxillofacial region and has a poor prognosis. Cytokeratin 19 (CK19) is a component of cytoskeleton protein. Previous studies have reported abnormal expression of CK19 protein in OSCC tissue. This study is to investigate the quantitative level of CK19 gene transcript in OSCC tissue as well as its clinical significance. Thirty-one OSCC patients (26 males and 5 females) took part in the present study, aged 34-78 years (mean 58.2 years). The level of CK19 mRNA was detected using fluorescent quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) in cancerous and paracancerous tissues. The relative quantification in cancerous tissue compared with paracancerous tissue was calculated using the 2(-DeltaDeltaCt) equation. The level of CK19 mRNA in cancerous tissue from OSCC patients was 2.21-fold higher than that in paracancerous tissue (P=0.020), and the amplicon was specific without genomic DNA contamination. The level of CK19 mRNA correlated significantly with the pathological differentiation grade of OSCC tissue (P=0.025), with poorer differentiation indicating a higher level of CK19 mRNA. These results suggest that fluorescent quantitative real-time RT-PCR is accurate and reliable for the detection of CK19 gene transcript levels in OSCC tissue. The level of CK19 mRNA was increased in OSCC tissue, and this was significantly correlated with the pathological differentiation grade.
口腔鳞状细胞癌（OSCC）是口腔颌面部最常见的恶性肿瘤，预后较差。细胞角蛋白19（CK19）是细胞骨架蛋白的一个组成部分。既往研究报道了CK19蛋白在OSCC组织中的异常表达。本研究旨在探讨OSCC组织中CK19基因转录本的定量水平及其临床意义。31例OSCC患者（26例男性，5例女性）参与了本研究，年龄34 - 78岁（平均58.2岁）。采用荧光定量实时逆转录聚合酶链反应（RT-PCR）检测癌组织和癌旁组织中CK19 mRNA的水平。使用2(-DeltaDeltaCt)公式计算癌组织与癌旁组织相比的相对定量。OSCC患者癌组织中CK19 mRNA的水平比癌旁组织高2.21倍（P = 0.020），扩增产物具有特异性，无基因组DNA污染。CK19 mRNA水平与OSCC组织的病理分化程度显著相关（P = 0.025），分化越差表明CK19 mRNA水平越高。这些结果表明，荧光定量实时RT-PCR检测OSCC组织中CK19基因转录本水平准确可靠。OSCC组织中CK19 mRNA水平升高，且与病理分化程度显著相关。
