Zhong Lai-ping, Chen Wan-tao, Zhang Chen-ping, Zhang Zhi-yuan
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007 Sep;104(3):377-84. doi: 10.1016/j.tripleo.2006.07.019. Epub 2006 Nov 7.
To investigate cytokeratin 19 (CK19) expression in oral squamous cell carcinoma (OSCC) tissue as well as its correlation with pathologic differentiation grade and prognosis.
Cancerous and distant tissues from 33 primary OSCC patients were collected during surgery. Each sample was divided into 2 parts, one for CK19 protein detection using immunohistochemistry, and the other for CK19 mRNA-level detection of relative quantification using fluorescent real-time reverse transcriptase polymerase chain reaction (RT-PCR).
The CK19 positive rate in cancerous tissue was 90.9% (30 out of 33) detected using immunohistochemistry, which was significantly higher than that in distant tissue (15.2%, 5 out of 33) by chi-square test (P < .001). The CK19 positive score in cancerous tissue was also significantly higher than that in the corresponding distant tissue by paired samples t test (P < .001). Patients with positive CK19 expression in distant tissue had higher tumor recurrence rate (P = .008) and lower survival rate (P = .015) than those with negative CK19 expression in distant tissue. Using fluorescent real-time RT-PCR, CK19 mRNA level in cancerous tissue was 2.21-fold higher than that in distant tissue (P = .020). The CK19 expression correlated significantly with pathologic differentiation grade, which was detected not only using immunohistochemistry (P = .002) but also using fluorescent real-time RT-PCR (P = .025), poorer differentiated indicating higher CK19 positive score and CK19 mRNA level.
Increased CK19 protein expression and gene transcription in OSCC tissue correlate significantly with pathologic differentiation grade. Positive CK19 expression in distant tissue suggests a higher tumor recurrence rate and a lower survival rate.
研究细胞角蛋白19（CK19）在口腔鳞状细胞癌（OSCC）组织中的表达情况及其与病理分化程度和预后的相关性。
手术过程中收集了33例原发性OSCC患者的癌组织和远隔组织。每个样本分为两部分，一部分用于免疫组织化学检测CK19蛋白，另一部分用于荧光实时逆转录聚合酶链反应（RT-PCR）相对定量检测CK19 mRNA水平。
免疫组织化学检测显示癌组织中CK19阳性率为90.9%（33例中的30例），经卡方检验显著高于远隔组织（15.2%，33例中的5例）（P <.001）。配对样本t检验显示癌组织中CK19阳性评分也显著高于相应的远隔组织（P <.001）。远隔组织中CK19表达阳性的患者比CK19表达阴性的患者具有更高的肿瘤复发率（P =.008）和更低的生存率（P =.015）。使用荧光实时RT-PCR，癌组织中CK19 mRNA水平比远隔组织高2.21倍（P =.020）。CK19表达与病理分化程度显著相关，免疫组织化学（P =.002）和荧光实时RT-PCR（P =.025）均检测到这一结果，分化越差表明CK19阳性评分和CK19 mRNA水平越高。
OSCC组织中CK19蛋白表达增加和基因转录与病理分化程度显著相关。远隔组织中CK19阳性表达提示更高的肿瘤复发率和更低的生存率。
