Ge Shujun, Pachter Joel S
Blood-Brain Barrier Laboratory, Department of Pharmacology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030, USA.
J Neuroimmunol. 2006 Aug;177(1-2):209-14. doi: 10.1016/j.jneuroim.2006.05.012. Epub 2006 Jun 27.
The isolation and culture of spinal cord microvascular endothelial cells (SCMEC), which form the blood-spinal cord barrier (BSCB), is described. Though morphologically similar to brain microvascular endothelial cells (BMEC) that form the blood-brain barrier (BBB), SCMEC express reduced amounts of several prominent BBB proteins, including tight junction-associated proteins ZO-1 and occludin, adherens junction-associated proteins beta-catenin and VE-cadherin, and the efflux transporter P-glycoprotein. These distinguishing features may reflect more widespread differences between the BBB and BSCB that impact physiological and pathophysiological processes.
本文描述了构成血脊髓屏障（BSCB）的脊髓微血管内皮细胞（SCMEC）的分离与培养。尽管SCMEC在形态上与构成血脑屏障（BBB）的脑微血管内皮细胞（BMEC）相似，但SCMEC表达的几种重要的BBB蛋白数量减少，包括紧密连接相关蛋白ZO-1和闭合蛋白、黏附连接相关蛋白β-连环蛋白和血管内皮钙黏蛋白，以及外排转运蛋白P-糖蛋白。这些显著特征可能反映了BBB和BSCB之间更广泛的差异，这些差异会影响生理和病理生理过程。
