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Evaluation of a commercial enzyme immunoassay for the detection of norovirus antigen in fecal samples from children with sporadic acute gastroenteritis.

作者信息

González G G, Liprandi F, Ludert J E

机构信息

Laboratorio de Biología de Virus, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Apdo. 21827, Caracas 1020-A, Venezuela.

出版信息

J Virol Methods. 2006 Sep;136(1-2):289-91. doi: 10.1016/j.jviromet.2006.05.024. Epub 2006 Jun 27.

DOI:10.1016/j.jviromet.2006.05.024
PMID:16806504
Abstract

The Ridascreen Norwalk-like virus enzyme immunoassay was compared with (RT)-PCR on 92 stool samples collected from children with sporadic acute gastroenteritis. Homogenization and pre-dilution of the whole stool sample resulted in high specificity (97.5%) and moderate sensitivity (60%). This assay may be useful to screen outbreaks for norovirus, but limited to detect the virus in sporadic cases of diarrhea.

摘要

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