Laboratory of Clinical Microbiology and Infectious Diseases, Isala Klinieken, Stilobadstraat 3, 8021 AB, Zwolle, The Netherlands.
Eur J Clin Microbiol Infect Dis. 2010 Jun;29(6):741-3. doi: 10.1007/s10096-010-0911-5. Epub 2010 Mar 21.
Worldwide noroviruses are an important cause of gastroenteritis and are major agents of both sporadic as well as epidemic infection. Because of the rapid transmission of the virus, early detection is essential. Until recently, the available test methods for the detection in stool were enzyme immunoassays and real-time reverse transcription polymerase chain reaction (RT-PCR), both of which take several hours to perform. We evaluated the rapid immunochromatographic test RIDA(R)QUICK Norovirus for the detection of norovirus in the stool of patients with acute gastroenteritis. This test is easy to perform and read and only takes 20 min. The sensitivity and specificity compared to RT-PCR results and the positive and negative predictive values were 57.1%, 99.1%, 93.3% and 91.2%, respectively. The rapid test is useful for quick screening, but a negative result should be followed up by RT-PCR.
诺如病毒是一种全球性的病原体，是导致肠胃炎的重要原因，也是散发性和流行性感染的主要病原体。由于病毒传播迅速，早期检测至关重要。直到最近，用于检测粪便中诺如病毒的方法是酶免疫测定和实时逆转录聚合酶链反应（RT-PCR），这两种方法都需要几个小时才能完成。我们评估了快速免疫层析检测 RIDA（R）QUICK 诺如病毒用于检测急性肠胃炎患者粪便中的诺如病毒。该检测方法易于操作和读取，仅需 20 分钟。与 RT-PCR 结果相比，该检测方法的灵敏度和特异性以及阳性和阴性预测值分别为 57.1%、99.1%、93.3%和 91.2%。快速检测方法对于快速筛查很有用，但阴性结果应通过 RT-PCR 进行后续检测。
