Lockhart K, McBeath A J A, Collet B, Snow M, Ellis A E
FRS Marine Laboratory, P.O. Box 101, Victoria Road, Aberdeen AB11 9DB, Scotland, UK.
Fish Shellfish Immunol. 2007 Mar;22(3):151-6. doi: 10.1016/j.fsi.2006.04.002. Epub 2006 Apr 25.
The Mx response was compared in parr and post-smolt Atlantic salmon following intra-peritoneal injection of the same dose of Infectious Pancreatic Necrosis Virus (IPNV) per g of fish. Mx gene expression, measured by quantitative RT-PCR in liver, showed a maximum level 3days after injection in parr with undetectable levels on day 7. In post-smolts, similar levels as in parr were attained on day 3, but levels then continued to rise on day 5 and 7 to about 10 times higher than the peak level in parr. Poly I:C injected parr showed Mx levels similar to IPNV injected post-smolts. Mortality from IPN in post-smolts occurred on days 6 and 7. Levels of IPN VP2 transcripts in parr were very low and did not increase with time, suggesting viral replication was low. Individual variation in levels of Mx and IPN VP2 gene transcripts was very high in post-smolts and although data is limited there was an inverse relationship between the levels of Mx and VP2, suggesting that individuals with high Mx levels on day 5 may be able to prevent viral replication. This contrasts with the response in parr, where IPN-resistance was not associated with a high Mx response.
对腹腔注射相同剂量传染性胰腺坏死病毒（IPNV）（每克鱼注射量相同）后的大西洋鲑鱼苗和成鱼的Mx反应进行了比较。通过定量逆转录聚合酶链反应（qRT-PCR）检测肝脏中的Mx基因表达，结果显示，鱼苗在注射后3天达到最高水平，第7天检测不到。在成鱼中，第3天达到与鱼苗相似的水平，但在第5天和第7天继续上升，比鱼苗中的峰值水平高约10倍。注射聚肌胞苷酸（Poly I:C）的鱼苗显示出与注射IPNV的成鱼相似的Mx水平。成鱼中IPN的死亡发生在第6天和第7天。鱼苗中IPN VP2转录本的水平非常低，且不随时间增加，表明病毒复制水平较低。成鱼中Mx和IPN VP2基因转录本水平的个体差异非常大，尽管数据有限，但Mx和VP2水平之间存在负相关关系，这表明第5天Mx水平高的个体可能能够阻止病毒复制。这与鱼苗的反应形成对比，在鱼苗中，IPN抗性与高Mx反应无关。
