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Primary structure of the cDNA 5'-terminal region encoding the N-terminal domain of the rabbit muscle alpha-actinin subunit.

作者信息

Jokhadze G G, Oleinikov A V, Nadirashvili N S, Zaalishvili M M

机构信息

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow.

出版信息

FEBS Lett. 1991 Sep 9;289(2):190-2. doi: 10.1016/0014-5793(91)81067-i.

DOI:10.1016/0014-5793(91)81067-i
PMID:1680752
Abstract

A number of cDNA clones have been obtained in summary encoding the N-terminal domain containing 286 amino acid residues of the rabbit skeletal muscle alpha-actinin subunit. Alpha-Actinin cDNA clones were isolated from specific cDNA libraries using the primer extension method for synthesis of the first cDNA chain. A strong stop signal for AMV reverse transcriptase in the 5'-terminal region of mRNA of alpha-actinin was found. It seems there is a G+C rich region (93% G+C nucleotides) including a continuous sequence of 23 G and C nucleotides encoding 6 glycine residues.

摘要

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