兔骨骼肌蛋白磷酸酶抑制剂2 cDNA的PCR克隆
PCR cloning of the cDNA of rabbit skeletal muscle protein phosphatase inhibitor-2.
作者信息
Zhang Z, Bai G, Lee E Y
机构信息
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101.
出版信息
Biochem Biophys Res Commun. 1992 Jul 31;186(2):1168-70. doi: 10.1016/0006-291x(92)90869-m.
PMID:1323285
Abstract
The coding region of the cDNA of protein phosphatase inhibitor-2 was determined by polymerase chain reaction amplification. The cDNA clone consisted of 621 nucleotides, and encoded 204 amino acids. The deduced amino-acid sequence was identical with that of the sequence reported by chemical sequencing methods.
摘要
通过聚合酶链反应扩增确定了蛋白磷酸酶抑制剂-2 cDNA的编码区。该cDNA克隆由621个核苷酸组成，编码204个氨基酸。推导的氨基酸序列与化学测序方法报道的序列相同。
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