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兔骨骼肌蛋白磷酸酶抑制剂2 cDNA的PCR克隆

PCR cloning of the cDNA of rabbit skeletal muscle protein phosphatase inhibitor-2.

作者信息

Zhang Z, Bai G, Lee E Y

机构信息

Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101.

出版信息

Biochem Biophys Res Commun. 1992 Jul 31;186(2):1168-70. doi: 10.1016/0006-291x(92)90869-m.

DOI:10.1016/0006-291x(92)90869-m
PMID:1323285
Abstract

The coding region of the cDNA of protein phosphatase inhibitor-2 was determined by polymerase chain reaction amplification. The cDNA clone consisted of 621 nucleotides, and encoded 204 amino acids. The deduced amino-acid sequence was identical with that of the sequence reported by chemical sequencing methods.

摘要

通过聚合酶链反应扩增确定了蛋白磷酸酶抑制剂-2 cDNA的编码区。该cDNA克隆由621个核苷酸组成，编码204个氨基酸。推导的氨基酸序列与化学测序方法报道的序列相同。

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PCR cloning of the cDNA of rabbit skeletal muscle protein phosphatase inhibitor-2.兔骨骼肌蛋白磷酸酶抑制剂2 cDNA的PCR克隆
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引用本文的文献

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Cloning and characterization of human phosphatase inhibitor-2 (IPP-2) sequences.人磷酸酶抑制剂-2（IPP-2）序列的克隆与特性分析
Mamm Genome. 1994 Aug;5(8):490-6. doi: 10.1007/BF00369318.
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Regulation of chromosome segregation by Glc8p, a structural homolog of mammalian inhibitor 2 that functions as both an activator and an inhibitor of yeast protein phosphatase 1.由Glc8p调控染色体分离，Glc8p是哺乳动物抑制剂2的结构同源物，它既是酵母蛋白磷酸酶1的激活剂又是抑制剂。
Mol Cell Biol. 1995 Nov;15(11):6064-74. doi: 10.1128/MCB.15.11.6064.